Can You Select For This Trait

[highgrade]

This strain has unknown heritage to me. Just some thing I let Mother Nature do her thing with on 21 sprouts I found at an abandoned grow site. I was wondering if the pistil color was something you can shoot for? 2 phenos appeared from the ones I selected out of 200. One has white pistils, the other has fuscia/purple, whatever you want to call it I guess. Cut a couple colas off and the aroma was amazingly close to that of a fresh cut Xmas tree.

Stay safe,
highgrade

[plantbuilder]

sure thing.... but you seem to be collecting the elite cuts before pollinating to preserve the gene pool.

you always manage to make my day, highgrade
peace
pb

[highgrade]

Thanks PB! Luckily I have a few thousand seeds to play with. Seems the more I read the more confused I get. Hopefully someday I'll find a mentor to guide me on the right path. Until then, thanks for your input as always bro.

Stay safe,
highgrade

[vapor]

in time it all becomes clear keep up the good work! i like pink pistals!

[highgrade]

Wouldn't preserving the gene pool be what I did originaly? I left all 7 males to pollenate 14 females. I collected the seeds from each female and have them seperated. From that group of seeds I started a couple hundred and only selected 8 that showed the most vigor due to lack of room (and balls) to grow out more. It was mainly just to test the strain and see if it was worth growing. Next step?????

Stay safe,
highgrade

[plantbuilder]

lol... awesome! (sorry i didn't quite read your first post properly)

lemme put the coffee on and we can discuss this.
yes you did exactly what i was hoping for. thanks for clarifying (you really did make my day yesterday)

now we enter a discussion... just to clarify you looked at 8 females out of 200 seeds and kept no males. they were flowered out and produced 2 keepers, but clones have not been kept. so now there is 800 beans of well preserved unknown f2 and we want to go hunting for wabbits (with the goal of producing the most keepers (pink, maybe both pink and white) per seedlot).

- we need to discuss your notes, assertations and assumptions about the 200 lot

- since so many candidates got scrapped (due to numbers, maintenance etc) i'm sure you can guess we will look at lowering the selection lot (ie. we need to get some frequency results) males, pink, white, short, tall, healthy, (notes, notes, notes)

ok... so how many beans you want to start provided we want to end up with something close to what happened previously down at the old abandoned mine... lol

love you highgrade
thanks for inspiring me to post
peace
plantbuilder

[Green Supreme]

YAY a re inspired PB. Now we're talkin. Peace GS

[plantbuilder]

i have some seeds similar to yours but i prolly only have 100 of them
they are from open pollinated plants that originally were sourced from an outdoor grower in the kootenays, the open pollination prolly involved about 35 total plants, i would say about 7-10 males but my seeds are not sorted like yours (if there are not sufficient notes or remaining bud samples that may not matter even)

the buds that provided the seeds were definitely the pine tree smell you describe, the pine also being represented in bud structure also

i'm sure you can see this being a benefit to outdoor and nature-managed plants
pine being not only pest control but rot control

just a few things i was thinking about on my morning walk
the sweetskunk inspired thoughts will have to wait until after the family dinner

q1: who is the one person on c-w that we want to help us here?

peace
pb

[highgrade]

Thanks for taking your time on this PB! I truly appreciate it.

1) The plants are still alive, 8 total from different seed batches collected.

2) It now appears there's 3 phenos.
a) dark green leaves with white pistils
b) dark green leaves turning purple with white pistils
c) light green leaves with purple pistils

3) I didn't take notes on anything:newbie: ( I do know what seed batch each is from)

4) All phenos are 10-12' tall. (makes it hard for me to get a good look)

5) I've only cut a few colas off so far because they're not done. These are labeled while drying.

I don't know the answer to Q1, sorry.

Here's some pics of what they look like presently minus the one already posted above.

1)Dark green leaves with white pistils
2)Light green leaves with purple pistils
3)Dark green leaves with white pistils
4)Purple leaves with white pistils
5)Purple leaves with white pistils

Thanks again PB and really do hope I can make your day again in the future.

Stay safe,
highgrade

[Parabola]

the short answer is yes, you can select for that trait . the frequency is most likely a huge variable at this stage, however. the problem again with cannabis males is their inability to express the desired traits.

good luck and nice plants man!

[highgrade]

the short answer is yes, you can select for that trait . the frequency is most likely a huge variable at this stage, however. the problem again with cannabis males is their inability to express the desired traits.

good luck and nice plants man!

Thanks! Well, got the ladder out for a closer look and it appears that the ones turning purple leaf also have close to the same pistil color. Not as vivid, but still have the tint.

Stay safe,
highgrade

[plantbuilder]

ok....

so there are 8 females that can still be hit with pollen (hopefully)
but no available donors from the unknown f2 generation(?)

do you plan on revegging these or is there a b/u cut for each?
which ones to reveg then? (min. 3 best)

basically you want to do three things:

-open-pollinate the selected f2 females (8 or less) from selected f2 males
-self the selected f2 females (and males)
-use unrelated males/pollen to outcross selected f2 females

i'm sure you want to ask if it's wise to just pollinate the pink-pistilled plants
i would pollinate the best showing of the eight and if 8 are great then all of them.
the same strategy should be applied to male selection.

there are some things that can be inferred about pink pistils and light green leaves from what we see happening, that they are likely to be recessive traits (but again this is generalizing)

we can and need to further examine the three tasks.

really ..i would keep all or the best of those 8 females and do then do the same thing with male populations

i would set your max amount of phenotypes per gender at 32 and the low end of course at 3

hope this helps some

peace
plantbuilder

[highgrade]

Thanks PB! I'll try and address each item as best as I can.

ok....

so there are 8 females that can still be hit with pollen (hopefully)
but no available donors from the unknown f2 generation(?)

Yes, but I'm not sure if it's too late to pollenate. Low 40s at night and humidity is hitting 87%. There are no available donors from the f2s, all were culled when they showed sex.

do you plan on revegging these or is there a b/u cut for each?

No b/u cuts for any. No plan to reveg any cause I don't do indoor any more. These things are huge to boot.


basically you want to do three things:

-open-pollinate the selected f2 females (8 or less) from selected f2 males
-self the selected f2 females (and males)
-use unrelated males/pollen to outcross selected f2 females

1)all males were culled
2)possible if I knew how with the females
3)possible, but the only pollen I have is the swt#3Xc99, also, it may be too late in the cycle

i'm sure you want to ask if it's wise to just pollinate the pink-pistilled plants
i would pollinate the best showing of the eight and if 8 are great then all of them.
the same strategy should be applied to male selection.

7 look great with #8 being the runt. The bud still looks really nice on her, so I'd say all of them.

there are some things that can be inferred about pink pistils and light green leaves from what we see happening, that they are likely to be recessive traits (but again this is generalizing)

Understood.

we can and need to further examine the three tasks.

really ..i would keep all or the best of those 8 females and do then do the same thing with male populations

Man, wish I could, but I don't see how.

i would set your max amount of phenotypes per gender at 32 and the low end of course at 3

For the next batch of seed germinated or this run? I really do think it's too late for this run, but I could be wrong.

hope this helps some

peace
plantbuilder

You, my friend, make more than just my day! Again, I appreciate you taking the time to run through this with me.

Stay safe,
highgrade

PS: Would it be better if I started over from scratch with seed stock?

[highgrade]

PB,

I think this run is past the point of being much use other than I got to look at a few phenos the seeds would produce. Sorry if I mislead you into thinking there was still room to play with this lot. All will finish in a week or two. Also, this was only a selection of 8 females out of the 14 different females I collected the seeds from because of all the culling I did.

In the next run would it be possible to use 10 seeds from each of the 14 batches? Or would I have to split it up into 20 seeds from 1-7 and do another run of 20 each from 8-14 for better numbers? Even these numbers would be pushing it for me as to room.

Stay safe,
highgrade

[plantbuilder]

no misleading done at all, (s'about to snow here)
that's the way we were going anyways
sorry i've been busy with midterms and trimming to pay for school
not my intent to leave you hanging
peace
plantbuilder

[Subzero420]

Thanks for taking your time on this PB! I truly appreciate it.

1) The plants are still alive, 8 total from different seed batches collected.

2) It now appears there's 3 phenos.
a) dark green leaves with white pistils
b) dark green leaves turning purple with white pistils
c) light green leaves with purple pistils

3) I didn't take notes on anything:newbie: ( I do know what seed batch each is from)

4) All phenos are 10-12' tall. (makes it hard for me to get a good look)

5) I've only cut a few colas off so far because they're not done. These are labeled while drying.

I don't know the answer to Q1, sorry.

Here's some pics of what they look like presently minus the one already posted above.

1)Dark green leaves with white pistils
2)Light green leaves with purple pistils
3)Dark green leaves with white pistils
4)Purple leaves with white pistils
5)Purple leaves with white pistils

Thanks again PB and really do hope I can make your day again in the future.

Stay safe,
highgrade Nice pic's those pink/purple pistils should make for a very good flavor & smell, by the way,, how "stinky" are they? or are they mild smelling? either way, those are nice.. I posted some pic's but they dont look as nice as yours do.. hats-off 2 U,,man.

[highgrade]

PB,

Good luck on your midterms bro! Glad you saw it that way also. You're not leaving me hanging at all, the damn change in season is.

Subzero420,

Thanks. As for smell, they're getting pretty bad. A buddy came over a few days ago and said he could smell it a block away. Real skunky. Today had 4 people walking down the river and heard one say 'yep, that's where it's coming from'. I expect a visit tonight, but the Catahoulas and electric fence work pretty good so far.

Stay safe,
highgrade

[Lungus]

PB,

Good luck on your midterms bro! Glad you saw it that way also. You're not leaving me hanging at all, the damn change in season is.

Subzero420,

Thanks. As for smell, they're getting pretty bad. A buddy came over a few days ago and said he could smell it a block away. Real skunky. Today had 4 people walking down the river and heard one say 'yep, that's where it's coming from'. I expect a visit tonight, but the Catahoulas and electric fence work pretty good so far.

Stay safe,
highgrade

[plantbuilder]

well I hope all is well highgrade,

so popped any beans yet? lol

if you are looking for keeper clones from the pink pistilled one's i would suggest starting 20-40 seeds. I find 20 is a good number searching while maintaining backups, but 10 is acceptable too. If you noted any pink pistils out of the original 14 down at the mine you should of course start those, but it's not crucial

if you are looking to continue breeding with the 'abandoned (gold) mine' to preserve and further increase the frequency of pink pistils, or rather successful plantys, then yes 10 of each one is fine. You can also start more of the anyone's that you knew to show desired traits; the key here is to not exclude healthy plants that do not. Everyone loves a good reacharound. Plus you will get lots of support for keeping some freaks in the mix. (the recessive breeding argument and also the reason we will discuss selfing desireable breeding stock). So start 100-200 beans just like you did last year but this time it's going to be a jizzfest (the innuendo will never end)

as stated earlier, this open pollination technique does not require the labelling of any seeds, it is all OGM f2.

if you wanted to do some select breeding, it would require labelling and maintaining backups. This whole thread started because we discussed (somewhat) in the other thread that you are throwing unknown traits out the door, while falsely claiming to preserve the expressed phenotypes.

So onwards we go...

p.s.
-thank you for the encouragement, my midterms went fine.

-you still think there are 3 pheno's after harvesting the 8 ladies? what can be inferred from this... it wasn't the first time those beans had been let to openly seed themselves down at the mine (highly possible)

-i won't call in the help yet... i always make sure to read anything chimera posts anywhere but he also has proprietary secrets to maintain... since hybe is nowhere to be found that i can see, and skunkman is just too cool for school (deservedly so). I'm pretty sure the guy i want to read all this crap has a day job at Tim Hortons (lol)... anywayz

the key here is to have fun and learn
your pics are truely amazing, and one day i aspire to have balcony dressings just like yours

peace
plantbuilder

[highgrade]

Glad things went well for you on the midterms PB!

Unless I set up a green house there won't be any popped until March.

-you still think there are 3 pheno's after harvesting the 8 ladies? what can be inferred from this... it wasn't the first time those beans had been let to openly seed themselves down at the mine (highly possible)

No. Shows what I don't know. I now believe there are different genotypes as well as a couple phenotypes of one.

I'm was planning on scouting out an area and getting some supplies in before the snow flies, but I missed my chance. First thing on the agenda will be that in the spring. I'll go with the 10 of each = 140:dig:

Am I correct in assuming that I should once again let them go with no selection process as to traits? Man, I sure hope nobody has something going close to this site!

Thanks again PB for your insight on this! Appreciated as always bro.

Stay safe,
highgrade