gojo
09-06-2008, 02:15 AM
Hey guys/gals,
As I'm sure most of you know, brassinolide has been shown for a long time that it offers many benefits to cultivation of p.cube when applied to a sub of horse dung/grain mix. Brassinolide should increase yield (in total fruit bodies and dry weight), speed of harvest, reduction in aborts, etc. And I assume the same benefits would be seen in h.poo/staw or just h.poo, or many other subs. But h.poo/grain was tested in the study. In the study by J.Gartz, G. Adam and H.-M. Vorbrodt they found that 10^(-2) ppm of brassinolide had strong positive effects when used with horse manure/grain as a substrate. That sounds a lot like spawning with colonized rye into h.poo...
10^(-2) = 0.01 ppm
To reach 0.01 ppm of brassinolide with 0.1% brassinolide use 0.038 grams per gallon of distilled water.
Here's the math I used to find the gram per gallon rounding to the thousandths place. I extrapolated the numbers from this page: http://www.super-grow.biz/Brassinolide01.jsp
.067 gram of 0.1% brassinolide in .871 gallon = 0.02 ppm
.871 gal / .067 gram = 13x
1 gal / 13x = .077 gram
.077 gram / 2 = .038 gram
.038 gram = 0.01 ppm of 0.1% brassinolide in one gallon of water.
Use of 0.1-1% brassinolide is most common in horticulture and stronger dilutions are prohibitively expensive. Besides, it's easy to reach 0.01 ppm with 0.1% brassinolide.
One could apply brassinolide to the water used when saturating h.poo prior to pasteurization. Or after pasteurization if additional moisture is needed with boiled distilled water. I have a bit of 0.1% brassinolide and I am going to apply it to all my h.poo sub this run :farm:
Here is a great source of 0.1% brassinolide, very inexpensive considering how little is required:
http://www.super-grow.biz/Brassinolide01.jsp
And some background info and FAQ on brassinolide:
http://www.super-grow.biz/Brassinolide.jsp
And finally a quote from "Growth-Promoting Effect of a Brassinosteroid in Mycelial Cultures of the Fungus Psilocybe cubensis":
In both cultivation systems the influence of 10^(-2) ppm brassinosteroid on growth of the fungus was investigated (ten runs each). In the experiments with agar we observed that growth of mycelia took place two to three times faster than in the control series without steroid.
In the dung/grain substrate the first flush of fruit bodies appeared remarkably earlier. Thus, whereas in the control the first sporocarps were produced within 4 to 5 weeks, the addition of the brassinosteroid caused a fruiting of the mycelia already in 3 to 3.5 weeks. Furthermore, a variation of the number of formed fruit bodies in the five flushes during fructification was observed, e.g., with brassinosteroid four to seven mushrooms were produced in the first flush compared to one to three in the control (Fig. 2). The steroid treated cultures afforded also a remarkably higher dry mass than the control cultivations (3.4 — 3.9 g versus 2.3-2.9 g/10 g substrate). The formation of incomplete fruit bodies, normally present in mushroom cultivation, was almost completely suppressed by the brassinosteroid. Interestingly, the added brassinosteroid also influenced the morphology of the formed fruit bodies which lacked the typical membranous annulus on the stems.
HTH :)
As I'm sure most of you know, brassinolide has been shown for a long time that it offers many benefits to cultivation of p.cube when applied to a sub of horse dung/grain mix. Brassinolide should increase yield (in total fruit bodies and dry weight), speed of harvest, reduction in aborts, etc. And I assume the same benefits would be seen in h.poo/staw or just h.poo, or many other subs. But h.poo/grain was tested in the study. In the study by J.Gartz, G. Adam and H.-M. Vorbrodt they found that 10^(-2) ppm of brassinolide had strong positive effects when used with horse manure/grain as a substrate. That sounds a lot like spawning with colonized rye into h.poo...
10^(-2) = 0.01 ppm
To reach 0.01 ppm of brassinolide with 0.1% brassinolide use 0.038 grams per gallon of distilled water.
Here's the math I used to find the gram per gallon rounding to the thousandths place. I extrapolated the numbers from this page: http://www.super-grow.biz/Brassinolide01.jsp
.067 gram of 0.1% brassinolide in .871 gallon = 0.02 ppm
.871 gal / .067 gram = 13x
1 gal / 13x = .077 gram
.077 gram / 2 = .038 gram
.038 gram = 0.01 ppm of 0.1% brassinolide in one gallon of water.
Use of 0.1-1% brassinolide is most common in horticulture and stronger dilutions are prohibitively expensive. Besides, it's easy to reach 0.01 ppm with 0.1% brassinolide.
One could apply brassinolide to the water used when saturating h.poo prior to pasteurization. Or after pasteurization if additional moisture is needed with boiled distilled water. I have a bit of 0.1% brassinolide and I am going to apply it to all my h.poo sub this run :farm:
Here is a great source of 0.1% brassinolide, very inexpensive considering how little is required:
http://www.super-grow.biz/Brassinolide01.jsp
And some background info and FAQ on brassinolide:
http://www.super-grow.biz/Brassinolide.jsp
And finally a quote from "Growth-Promoting Effect of a Brassinosteroid in Mycelial Cultures of the Fungus Psilocybe cubensis":
In both cultivation systems the influence of 10^(-2) ppm brassinosteroid on growth of the fungus was investigated (ten runs each). In the experiments with agar we observed that growth of mycelia took place two to three times faster than in the control series without steroid.
In the dung/grain substrate the first flush of fruit bodies appeared remarkably earlier. Thus, whereas in the control the first sporocarps were produced within 4 to 5 weeks, the addition of the brassinosteroid caused a fruiting of the mycelia already in 3 to 3.5 weeks. Furthermore, a variation of the number of formed fruit bodies in the five flushes during fructification was observed, e.g., with brassinosteroid four to seven mushrooms were produced in the first flush compared to one to three in the control (Fig. 2). The steroid treated cultures afforded also a remarkably higher dry mass than the control cultivations (3.4 — 3.9 g versus 2.3-2.9 g/10 g substrate). The formation of incomplete fruit bodies, normally present in mushroom cultivation, was almost completely suppressed by the brassinosteroid. Interestingly, the added brassinosteroid also influenced the morphology of the formed fruit bodies which lacked the typical membranous annulus on the stems.
HTH :)