Growing P.cubensis...strains, teks and kit info and a ? [Archive]

gojo

04-30-2008, 10:02 AM

Hey yo'

OK, well it's getting beautiful, warm and sunny outside again...time to trip!!! :kitty: :loco: This year I want to grow my own fungi, and besides, where I live I will be my only and the town's only connection.

I'm gonna go with:
B+
Ecuador
Golden Teacher

I grew B+ years ago but I had contamination problems and had poor pinings. I tried the cake tek with wide mouth mason jars, brown rice flour, vermiculite and perlite. Jars are pressure cooked, filled and pressure cooked again before they are inoculated with 5-10cc of spores.

Here's a good guide for the cake tek: Magic mushroom growing for dummies v1.0 (http://www.magic-mushrooms.net/mmg4dummies/Magic_mushroom_growing_for_dummies.html)
All parts for 10 cakes will cost about $40.00 or $80.00 if you buy a canning pot. The cake tek is the way most people grow but you don't get a good pining until the 2nd or 3rd casing of the cake which will take about 2-3 months.

NOte:
1) All ingredients should be organic, that guide is old!

2) I suggest when you grind your brown rice you use a coffie bean grinder, you can get a hand-held electric one for about $30.00.

3) I like to wash all the pots, pans, bowls, utensils, etc I'm going to use in the dishwasher with an all natural dish washing soap ("7th Generation" is good). After they are washed I keep them covered and/or open side down on a clean counter to limit contamination.

4) In steps 6-9 (under "steps to take") I suggest you use a a "Water Bath" or "Canning Pot" (http://www.mycosupply.com/cgi-bin/shopper.cgi?preadd=action&key=CANN02) for about $40.00. On the more expensive side a pressure cooker ($180.00) or even a steam sterilizer ($600.00) work well. Any of the four are way more effective and easier to use than the guide.

5) In step 10 I suggest wiping your hands and arms past the elbows with rubbing alcohol after washing with anti-bacterial soap to be extra careful. I also use a dusk mask and a hat to prevent my germs from ruining the grow.

6) In step 10 when you inoculate the jars I suggest you do it in a bathroom because they are small and you'll have less contamination. I clean he counters, mirrors, toilet, etc with anti-bacterial cleaner and then I wipe things down with rubbing alcohol. Finally I spray the bathroom with lysol and close the door for an few hours to disinfect the room before I inoculate the jars in the bathroom.

7) In step 16 I would suggest using a fish tank aquarium (aka "fruiting chamber") with a lid because you can see them grow! (10gallon tank for $20.00). You should transfer the cakes from the jar into the fruiting chamber while inside the bathroom. The fruiting should take place in the bathroom to limit contamination.

8) In the US spores are legal because they are for scientific study (microscopic use), except in ID, CA, and GA. This is why you can buy spores from US companies over the interent, spore possession is a legal loophole in the US. But, you ARE NOT allowed (at least legally) to inoculate and grow the spores within the United States :point: (yea right!)

Links:
A) Online info source (but a bit convoluted):
http://www.shroomery.org

B) Sources of spore syringes, spore prints, spawn bags, kits, etc:
http://www.shroomery.org/sponsors.php

C) A spore source for my Canadian friends :whisper: :
http://www.ralphstersspores.com/index_files/Page5926.html

D) Another spore source in Canada (spores are on back-order!):
http://www.fsrcanada.com/

:confused: I remember a guy who was on the original CW who sold growing kits he made out of tupperware. Anyone know who I'm talking about, is he still around somewhere?

This time around l'd like to find a more user friendly method for higher yields. I'll do the cake or double cake tek if it's the best way to go. Anyone have experience with larger terrariums or kits?

I'm very open to suggestions on strains, teks, kits, etc, etc,

So who is growing the fungi? Got pics? :photo:

dpn

04-30-2008, 11:37 AM

Heres a pic of a cambodian. I first started with the wild bird seed tek but had problems with wet spot. So i went back to the brown rice flour tek but i would use 750ml jars with special lids with self healing injector ports, so i could shake the contents so the spores would spread evenly and colonize quickly... it worked great, then i would break up the cake and would spawn it to pasteurized horse poo (using agars tek) i had more mushies than i could possibly consume in a year... if interested i can go into more detail, let us know dpn....

dpn

04-30-2008, 11:43 AM

oh and i would definately spawn to horse poo, from my experience the pf tek gave me poor yields...
http://www.shroomery.org/8397/Horse-poo-substrate-preparation Agars horse poo tek
http://www.shroomery.org/forums/showflat.php/Number/6381868#6381868 One way to do the lids, without having to worry about the verm barrier...

Green Supreme

04-30-2008, 07:30 PM

I swiped this from another site. hope its somehow helpful. Peace GS

It has been over half a decade since I have grown mushrooms and just as long since I have eaten them, however I was talking witha friend in chat and told them that I would post up this formula. I decided to post it here in the forums instead of just chat to possibly help out some other folks. This is s formula given by the man, Paul Stamets himself, and if you don't know who that is then you shouldn't even be reading this thread let alone growing fungi. He personally gave his unpublished personal formula for fruiting out Psilocybe cubensis on the pf-tek style cakes and a mushroom conference prolly a decade back. Anyways here it is.

PS. Always make sure your source is completely organic and not treated with pesticides or fungicides for obviously reasons. Also get all ingredients whole and grind into powder yourself, I use a coffee grinder.

5 Cups Vermiculite

1 & 1/2 cups brown rice flour

1/2 cup ground Rye grain

1/3 cup Finch seed

2 & 1/4 cup distilled water

This will make the same thing as the pf-tek style cakes only with better nutrient base which equals into not only more, but stronger mushrooms. I hope this helps a few of you. Peace

gojo

04-30-2008, 07:45 PM

Hey bro!! :D

Hope you don't mind but I'm gonna pick your brain :yapyap:

Heres a pic of a cambodian

Nice! How is the Cam? I've read it can be very potent or med potent. I'm looking forward to trying the Ecuadorian, supposed to be real potent. The Golden Teacher is supposed to yield very well and is med potent too but it doesn't colonize very quickly.

so i could shake the contents so the spores would spread evenly and colonize quickly

what mix do you use for your cakes? Do you use perlite and vermiculite? So after you inoculate the jars you shake them a few times a day? hummm, that is probably very helpful for gas exchange in the jar too! GOOD IDEA.

How full are your jars, 1/4 full with cake?

I figured out what "TYVEK" is but what the heck is "ACL" in terms of "ALC dipped micropore tape", is it just a type of micropore tape?

oh and i would definately spawn to horse poo

I have access to fresh horse poo so I guess to age it I can spread it out and let it dry out? I think i'll also put the poo into my food processor to break it up really well before I soak it.

Do you reuse your poo that has been spawned on? YOu can get multiple fruits out of one poo terrarium right? Do you simple add new cakes?

What did you use as a fruiting chamber (terrarium)? How big was the fruiting chamber? DId you need to control humidty? I know CO2 is the enemy of fungi, did you just open the terrarium once a day to let the Co2 escape? (this seems like it may cause contamination)

19. In aseptic conditions, dump (however much) into SANITIZED TRAY & add spawn ASAP (20% + spawn rate).

So the "sanitized tray" would be the terrarium fruiting chamber right? And "spawn" would be a colonized cake right? But what does "20% + spawn rate" mean? :confused2:

If interested i can go into more detail, let us know dpn....

Yea please!!!

I can't wait to start, should be in about a month...I'll do a thread about it. Maybe you can help me in case I fuc* things up in a major way? :o

gojo

04-30-2008, 07:49 PM

Hey GS!

I swiped this from another site. hope its somehow helpful. Peace GS

YES! Paul Stamets is the man!

Also get all ingredients whole and grind into powder yourself, I use a coffee grinder.

THis is how I originally made my cake ingredients. I bought whole brown rice and grounded it into a powder, maybe I should have ground it longer.

This will make the same thing as the pf-tek style cakes only with better nutrient base which equals into not only more, but stronger mushrooms. I hope this helps a few of you. Peace

NICE!!!

dpn

04-30-2008, 09:44 PM

hello, yes ive found the cambodians to be quite strong and i was surprised how much i felt 1 dried gram :fly: ralphster is great vendor. Ecuadorians, :) i always wanted to try, supposed to be very visual.
I just use the standard brf recipe they all should be similiar and i always made my own fresh flour i think i heard its better nutritionally too as its been less exposed to the air + i would make 20% approx of the mix slightly coarse as this helps keep the mix less compact and helps when you shake. When adding water to the mix its best to moisten the verm first with the water and then add the brf as this keeps the mix light and fluffy.
I would fill the the (750ml) jars about 2/3rds full trying to get the mix to slope at 45% or as much as possible to stop the mix sticking/clumping together after its been pressure cooked (rather than just dumping it flat and level like you would normally).
I shake the jars as soon as the pressure cookers cool enough to open, to separate any clumps that may have formed during sterilization, next shake comes after i inject the spores into the jar to help spread the myc as evenly as possible. The last shake comes after about 25% has colonized, thats if im unhappy as to how many points the mycelium has started from, you can miss this stage iif desired.
ALC = alcohol, i use the alc wipes as a protective blanket to inject into the tape.

No need to blend the poo lol its good to have that texture for the mycelium networks to navigate through. You can get many flushes off of a good poo cake :) i would start fresh after i feel the cake has been spent and all the nutrients have been consumed.
My terranium was a big transparent rubber maid like container (2.5 x 1.5 x 1.5?) with about 4 inches of perlite soaked and drained to keep it humid, i opened and fanned the box 5x a day? while misting with my misty mate simultaneously... carbon dioxide (stagnation) is the culprit.
The brf & verm once fully colonized is added to the freshly pasteurized poo and combined together at ratios of 20%+ brf & verm to 80% poo
The reason for the + sign is because its prefered for beginners to start out at 30% to speed up colonization and try and beat the risk of contamination.
i hope this helps, anymore questions dont be shy :)

shadowlord

04-30-2008, 10:31 PM

Thanks for the links and the info. Have done one run of Cubensis b/4 and am ordering a syringe to 'Nocc up some jars to do a PF Tek this time around.
I will post up Pictures once I am under way and see what kind of interest there is here in Mycology and Psychedelics in general!

To Fathom Heaven or Hell just take a pinch of Psychedelics.

P.S. Thanks for the recipe for 1/2 pint jars G.S. Just the size I happened to get for the occasion!

Springs

05-01-2008, 12:05 AM

gojo

05-01-2008, 02:19 AM

hello, yes ive found the cambodians to be quite strong and i was surprised how much i felt 1 dried gram :fly: ralphster is great vendor.

Nice, maybe I'll get some Cambodian instead of B+. The B+ is good for beginners as it can grow in the widest variety of conditions. The problem with B+ is they aren't real strong or and dont' yield as well as others. Especially the Golden Teacher and Ecuadorian.

I would fill the the (750ml) jars about 2/3rds full trying to get the mix to slope at 45% or as much as possible to stop the mix sticking/clumping together after its been pressure cooked (rather than just dumping it flat and level like you would normally).

good tip!

ALC = alcohol, i use the alc wipes as a protective blanket to inject into the tape.

so you just put a pice of alc wipe over the micropore tape which is over the Tyvek?

My terranium was a big transparent rubber maid like container (2.5 x 1.5 x 1.5?) with about 4 inches of perlite soaked and drained to keep it humid,

So the perlite is a bottom layer? and you put the poo on top of it?

i hope this helps, anymore questions dont be shy :)

Thanks!

gojo

05-01-2008, 02:23 AM

Thanks for the links and the info. Have done one run of Cubensis b/4 and am ordering a syringe to 'Nocc up some jars to do a PF Tek this time around.

What variety did you grow before and what did you think about it? What varieties are you going to buy this time and why?

P.S. Thanks for the recipe for 1/2 pint jars G.S. Just the size I happened to get for the occasion!

I hipe you got the wide-mouth kind that slope outward.

Looking forward to the pics!

Green Supreme

05-01-2008, 02:40 AM

Go Team Shroom Go. Large pizzas for everyone. Peace GS

gojo

05-01-2008, 02:47 AM

Nice, got to love the higher fungi.

Just getting things into gear for cultivating the shrooms myself, aswell.

Sweet!!!

This is great, we have 4 people who are going to post their fungi grows, excellent! More the merrier, anyone else want to join this group of "fun guys"!?! (and gals I'm sure ;) )

Decided to try a few strains this time, ordered some Equador, Orissa India, Malabar(India), Koh Samui Super(Thai), Syzygy(TM iso), Panama, Na Muang(thai), all from ralphsters.(syringes)

Sweet! I gonna do 20 jars and 2-3 terrariums so I'm now thinking about more varieties too. There are just so many available, heck, sporeworks has like 57 different varieties!

I was thinking about the Panama too. Why did you choose those other strains? I'm gonna check out ralphsters stock, they've got over 89 varites, holy crap!

Inocced to LC's and agar, currently incubating on rye and millet.

Could you please go into more detail about your use of agar and what's an "LC"? (sorry for the possibly dumb question)

Plan on spawning to a bulk mix of poo's and straw, focusing solely on "mono tubs"

I like the neglect aspect of the mono tub, no fanning, no misting = less chance for contam and more time to focus on producing spawn.

Could you please also go into further detail about "mono tubs"? what are they? I like the idea of no opening too.

Ive also got some wild oyster spores, my first go with the gourmets.

:shocked: You mean there are non-psychedelic mushrooms? :allgood2:

Ill post when the spores are flying :fly:

Great! Maybe if there's enough interest (and I suspect there is!) all of us could ask C-ray really nicely and he'll make a sub-forum for fungi in the "Lancifer's Plants" forum?
:bump:

gojo

05-01-2008, 02:55 AM

Ok, well I'd like to start a little list of vendors who we have USED and you can vouch for...

NOte: If this turns into a "vendor" war like it would with seed vender's that would be really lame!

I can vouch for:

Sporeworks (http://www.sporeworks.com)

Little Shop of Spores (http://www.lilshopofspores.com/)

The Hawks Eye (http://www.thehawkseye.com/)

DPN and Springs both vouch for:

Ralphsters Spores (http://www.ralphstersspores.com/)

Ralphster Spores in Canada (http://www.ralphstersspores.com/index_files/Page5926.html)

Any others?

gojo

05-01-2008, 03:24 AM

Some interesting tools:

Magic Air Exchange System (http://www.mushroommagic.com/maes.htm)

http://img241.imageshack.us/img241/6857/bubblersetsmallok0.th.jpg (http://img241.imageshack.us/my.php?image=bubblersetsmallok0.jpg)

This could be useful if one has issues with contamination. It looks like it can be rigged easily into a tuppaware container. It's $70.00 but it seems a waste if you can grow good fungi without it.

Air exchange. Until now this has been the most difficult part of the fruiting process. Mushroom mycelium produces CO2 (carbon dioxide). CO2 is heavier than air and stays on top of the casing layer. Mushrooms will not grow through this layer of CO2, which must be removed for your mushrooms to grow.

Prior to the introduction of the Magic Air Exchange System this layer of CO2 was vented by opening the unit and fanning the CO2 out by hand two or three times a day. This exposed the system to airborne contaminants.

We had experimented for years using a simple air pump to introduce the small amount of required fresh air into the systems. Unfortunately, there was no practical way to control the quality of this air. It was just pushing in unfiltered air along with any airborne contaminants. We experimented with our own filters and they either inhibited airflow or were not effective at contaminant removal.

Recently, we found a company that makes laboratory quality, inline air filters. These filters are used in labs to provide air for sterile culture work. They filter contaminant particles down to .20 microns. Our tests showed this filter to be perfect for introducing pure air into Magic Kits for CO2 displacement.

Mushroom kits and greenhouses!! (http://www.mushroommagic.com/magickits.htm)

http://img241.imageshack.us/img241/7660/greenhousexz0.th.jpg (http://img241.imageshack.us/my.php?image=greenhousexz0.jpg)

I'm thinking about getting the "Magic Master System (http://www.mushroommagic.com/master_system.htm)" someday, maybe after this summer. I just going to put it in a spare bedroom and grow all winter!

Magic introduces the Magic Master System - A10
The ultimate growing system for mushrooms...all in an area less than 10 square feet. You can be on your way to growing in about a week after you place your order!

Negative Ion Generator (http://www.mushroommagic.com/ion.htm)

http://img241.imageshack.us/img241/9071/ionizerleftoq2.gif (http://imageshack.us)

Good for defeating contamination in greenhouse, bigger terririums:

This great little device emits millions of negative ions per second. These combine with contaminants, which become negatively charged and attracted to the positively charged collector base.The generator also produces a gentle stream of air that helps remove CO2 from your Magic Greenhouse. And it produces a positive air pressure to keep contaminants from entering. Covers 1500 cu. ft.

gojo

05-01-2008, 03:25 AM

Go Team Shroom Go. Large pizzas for everyone. Peace GS

ummm...pizza...im hungry! I'll take mine with mushrooms! :D

islandgrow

05-01-2008, 04:22 AM

Hey Gojo I tried to grow Treasure coasts last summer and failed, but I can give advice if you need any. I did the martha tek but I kept getting contamination problems. A clean space and a good pressure cooker are key, as is a glove box.

gojo

05-01-2008, 04:32 AM

Hey IG,

Sure, I'd love you hear your input. So whats the "martha tek"? Can you explain it or do you have any links?

Whats the deal with the "Treasure coast", is that's it's name at all the banks or is it called something else?

ARe you gonna try and grow again? You should start up with the rest of us...it would be cool to get a bunch of people growing together! :grouphug:

later!

gojo

05-01-2008, 04:56 AM

Hey again!,

Got some links for everyone from shroomery.org:

1. "Strain Discussion Thread" (http://www.shroomery.org/forums/showflat.php/Cat/0/Number/7955073/an/0/page/0)

2. "Fahface' very own Strain Library...with Pics" (http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8106067/an/0/page/0) <-- This is great!!!

3. "my favorite reads "tek"" (http://www.shroomery.org/forums/showflat.php/Number/8100876#8100876)

4. "Where can I find.. Where do I get.. I need.. Advice!!"
(http://www.shroomery.org/forums/showflat.php/Number/8053712#8053712)

:D

Springs

05-01-2008, 05:02 AM

I was thinking about the Panama too. Why did you choose those other strains? I'm gonna check out ralphsters stock, they've got over 89 varites, holy crap!

No real reason, basicly a crap shoot, id like to collect the unique expresions, but for the most part p.cubes are the same. My next purchase will be some p.azure's and pan and p. cyan's(wood decomposers) they produce more active chemicals :) I have grown mazatec, pink buffalo and brazilian in the past aswell, all great strains, I may have maz and pb in spore form aswell, have to try and germinate them.

Could you please go into more detail about your use of agar and what's an "LC"? (sorry for the possibly dumb question)

The only dumb question is the one not asked ;)

LC is a liquid culture, its a nutritional water mixture to culture pure mycelium, used to inoculate spawn(grains and flour mix) its a quick way to colonize your spawn, instead of injecting spores you inject mycelium, knocking a few days off incubation.

Im using agar as gelatinous medium in a nutrient mix poured into petri dishes, making sure to use proper sterile procedures. Its my first time playing with agar and petri's, they can be used for many purposes, such as isolating clean mycelial growth from a wild or contaminated spore print. or for isolating spore germination over mulispore germ. Agar work is not required at all, its considered more for advanced hobbiest.

Also agar isolates usually then get transfered to a LC or straight to spawn depending on prefrence.

Could you please also go into further detail about "mono tubs"? what are they? I like the idea of no opening too.

Ill take and post some pics once I construct my first tub, its basicly a rubbermaid with holes in side at semi specific spot covered or filled with your choice of filter material. It is designed for bulk substrates, as a bulk substrate fully and properly colonized will create its own environment, in terms of humidity, and as for c02 half of the holes in the chamber are at the same spot as the top of the substrate, the effect is c02 produced falls out of the holes as fresh air is brought in from the other half of the holes near the top of the chamber.

Check out a few logs at theshroomery.org where she has evolved, many people use it. Its the best.

Ill post a few variations of making them and we'll all find out which performs the best :D

:shocked: You mean there are non-psychedelic mushrooms? :allgood2:

So many with so many properties it boggles the mind, cure cancer with that one, kill a man with the other.

Read, listen and watch Paul Stamets, he's a smart one.:old:

islandgrow

05-01-2008, 05:09 AM

This is a ruff martha tek I modified it a lot. Its basically a sealable shelf that you grow the mushrooms in, for large scale. I took a 20 gallon bin and got a fog generator the kind they use in some of the newer cloners. I put it in the bin with the water and then attached a fan to the top of the bin and a tube from the 20 gallon bin to the other bin(where the shrooms where to be grown) where I had cut a hole for the hose and it would push the fog in. The advantage of this tek over others is the fog provides fresh air and humidity. Heres the super cool part. I put a water heater in the 20 gallon tub and got the water to like 79 degrees so that 20 gallon bin was regulating temp,humidity,freshair. All in one bin. The thing had perfect temp/humidity always.

TC are a strain that is heavy fruiting but poor colonizing. The strain is supposed to be VERY visual.

Springs

05-01-2008, 07:06 AM

A good shroomery link: http://www.shroomery.org/forums/dosearch.php?namebox=135712 (Agar's posts)

:good:

dpn

05-01-2008, 02:42 PM

i use the alcohol wipe as a blanket really just to create like a cover when i inject into the tape and the alc will sterilize the stem of the needle as it goes into the jar... I use stainless steel containers to put the brf + verm cake and poo combination into this sits on top of the perlite... you may want to resoak the perlite once or twice week to keep it fresh, you could use a bit of hydrogen peroxide too...

Heres a pic of stainless steel container, imagine this filled with colonized horse poo + brf & verm (spawn) all white with mycelium, and full off mushrooms... you can get a healthy amount of shrooms and this is the way i would do it, personally i wouldn't waste my time with the pf tek, instead use those cakes as a spawn to horse poo. Its not hard and my first attempt i was more than happy with yields, but its up to you good luck with whatever you do.

ps you can see a 250ml jar next to a 500ml jar with my special lids (doritos are good as they have a nice rubber seal inside, and i like salsa) i was actually using 750ml jars but i think they accidently got recycled?

dpn

05-01-2008, 02:46 PM

i picked that stainless steel container from a car boot sale for a £5 ($10) there about 6x amount new btw :)

islandgrow

05-01-2008, 05:55 PM

if I could do it over again, I would start with the cakes, but spawning it to a substrate produces a much better yield. Nice little set up you got there DPN.

dpn

05-01-2008, 06:01 PM

The idea that psilocybin (active part of the mushroom) is 4-PHOSPHORyloxy-N,N-dimethyltryptamine and (taken from occult principles of health)

Phosphorous is the particular element by means of which the Ego is able to express thought and influence the dense physical body. It is also a fact that the proportion and variation of this substance is found to correspond to the state and stage of intelligence of the individual. Idiots have very little phosphorus; shrewd thinkers have much; and in the animal world, the degree of consciousness and intelligence is in proportion to the amount of phosphorus contained in the brain.

It is therefore of great importance that the aspirant who is to use his body for mental and spiritual work, should supply his brain with the substance necessary for that purpose. Most vegetables and fruits contain a certain amount of phosphorus, but it is a peculiar fat that the greater proportion is contained in the leaves, which are usually thrown away. It is found in considerable quantities in grapes, onions, sage, beans, cloves, pineapples, in the leaves and stalks of many vegetables, and also in sugar-cane juice, but not in refined sugar.

The following table shows the proportions of phosphoric acid in a few articles:

100,000 parts of:

Barley, dry, contains of phosphoric acid.........210 parts

Beans ...........................................292 "

Beets ...........................................167 "

Beets, Leaves of.................................690 "

Buckwheat........................................1 70 "

Carrots, dry.....................................395 "

Carrots, Leaves of...............................963 "

Linseed..........................................8 80 "

Linseed, Stalks of...............................118 "

Parsnips.........................................1 11 "

Parsnips, Leaves of.............................1784 "

Peas ...........................................190 "

... when i take mushrooms my intelligence/consciousness/spirit is magnified greatly, the wonders of phosphorus :old:

dpn

05-01-2008, 06:04 PM

I like the sound of your set-up IG any chance of a pic? as i wanted to make one very similiar to the one you made...

shadowlord

05-01-2008, 08:11 PM

Nice, got to love the higher fungi.

Just getting things into gear for cultivating the shrooms myself, aswell.

Decided to try a few strains this time, ordered some Equador, Orissa India, Malabar(India), Koh Samui Super(Thai), Syzygy(TM iso), Panama, Na Muang(thai), all from ralphsters.(syringes)

Inocced to LC's and agar, currently incubating on rye and millet.

Plan on spawning to a bulk mix of poo's and straw, focusing solely on "mono tubs"

I like the neglect aspect of the mono tub, no fanning, no misting = less chance for contam and more time to focus on producing spawn.

Ive also got some wild oyster spores, my first go with the gourmets.

Ill post when the spores are flying :fly:

Let me know how your mono-tub deal works out for you. I am going to do smaller PFTek in a terrarium this time as I tried the monotub and got one small flush and then the second better one got ruined about half way through due to contams. But then, it is a moldy climate here in the PNW so contams are often a problem.
I'll also post up my pics of my cacti collection. 'Shrooms are nice but mescaline/ cactus tea is far better. Best yet would be some good old Lucy, but there all good!

gojo

05-01-2008, 08:21 PM

I'll also post up my pics of my cacti collection. 'Shrooms are nice but mescaline/ cactus tea is far better.

Cool! I've been wanting to grow some cacti (peyote) too but the 5-7 year grow time gives me pause. Though I like how you can graft a young peyote cacti (1 year) to a faster growing cacti (non-psychedelic) and harvest buttons in 3 years!

I've got to get growing some cacti, its been on my to do list for years. I've never eating peyote or mescaline (micro-dots), what's the trip like?

Best yet would be some good old Lucy, but there all good!

What is Lucy?

dpn

05-01-2008, 08:24 PM

lucy in the sky with diamonds, acid :smile2:

shadowlord

05-01-2008, 09:39 PM

Cool! I've been wanting to grow some cacti (peyote) too but the 5-7 year grow time gives me pause. Though I like how you can graft a young peyote cacti (1 year) to a faster growing cacti (non-psychedelic) and harvest buttons in 3 years!

I've got to get growing some cacti, its been on my to do list for years. I've never eating peyote or mescaline (micro-dots), what's the trip like?

What is Lucy?

You don't have to wait years for your buttons to grow. There are sites where you can order Peyote ( Lophophora Williamsii ), or try San Pedros or Peruvian Torches ( Trichocereus Pachanoii or Peruvianus respectively ) they are legal to own and cultivate. They are sold as ornamentals or you can order cuttings online to make tea or extract the mescaline therefrom.
As to the trip; I'll let you know. SWIM is about to have their first experience w/ cactus tea here shortly. Mescaline is supposed to be very comfortable and natural and supposed to be more spiritually profound than LSD but we will see about that LOL!

gojo

05-01-2008, 09:56 PM

You don't have to wait years for your buttons to grow. There are sites where you can order Peyote ( Lophophora Williamsii ), or try San Pedros or Peruvian Torches ( Trichocereus Pachanoii or Peruvianus respectively )

So they are a few years old already? Do you grow them further or just harvest the buttons?

Personally though, becuase the high is so spirtual I want to grow my own from seed...in harmony with the plant type of hippy thing. I can wait a few years to grow from seed and graft I think...I'll have plenty of 'shrooms till then!
:woohoo:

they are legal to own and cultivate.

Not in the US, heck, even the Peyote church has been raided and non-Indian members arrested!

They are sold as ornamentals or you can order cuttings online to make tea or extract the mescaline therefrom.
As to the trip; I'll let you know. SWIM is about to have their first experience w/ cactus tea here shortly. Mescaline is supposed to be very comfortable and natural and supposed to be more spiritually profound than LSD but we will see about that LOL!

Nice, let use know!
:D

Springs

05-01-2008, 10:42 PM

Let me know how your mono-tub deal works out for you. I am going to do smaller PFTek in a terrarium this time as I tried the monotub and got one small flush and then the second better one got ruined about half way through due to contams. But then, it is a moldy climate here in the PNW so contams are often a problem.
I'll also post up my pics of my cacti collection. 'Shrooms are nice but mescaline/ cactus tea is far better. Best yet would be some good old Lucy, but there all good!

Ive used my own variant of a "monotub" before it caught on, it was a way cruder less efficent version, and it worked awsome with spawning to straight straw, 5 inch sub depth biggest single shroom was 10g dried, got 5 flushes from that particular tub.

Contams are inevitable imo, but can be controlled to a point of not being a problem.

Ill start my log soon, just grain to grain transfered 4 quarts of panama to 7 - 5lbs spawn bags, next step aquire bulk substrate asap and pasturize!

shadowlord

05-01-2008, 11:05 PM

So they are a few years old already? Do you grow them further or just harvest the buttons?

Personally though, becuase the high is so spirtual I want to grow my own from seed...in harmony with the plant type of hippy thing. I can wait a few years to grow from seed and graft I think...I'll have plenty of 'shrooms till then!
:woohoo:

Not in the US, heck, even the Peyote church has been raided and non-Indian members arrested!

Nice, let use know!
:D

From Seed is very personal and how I will do the Peyote which is indeed illegal. Although the Native American Church should not be getting raided as they are allowed it as their religious sacrament.
What I referred to as being legal were the San Pedro and Peruvian Torches. There are many other Trichocereus species that have the Magic Alkaloid in them and they are legal as ornamental plants. However, if they caught you making mescaline or selling them for consumption is, indeed, illegal.
They will have to make everything illegal and lock up the whole world. There are so many entheogenic plants and substances in the world. But the Main Cop won't be happy until everyone is locked up and then He can lock himself up and everyone will finally be safe. We're all caught in some weird power mad trip.

gojo

05-01-2008, 11:16 PM

What I referred to as being legal were the San Pedro and Peruvian Torches. There are many other Trichocereus species that have the Magic Alkaloid in them and they are legal as ornamental plants. However, if they caught you making mescaline or selling them for consumption is, indeed, illegal.

Ah! That explains it nicely for me thanks! I'm sooo gonna start growing some, or all 3!

islandgrow

05-02-2008, 04:18 AM

There it is DPN ask me if you have any questions. I cut out a larger section of the top for a light, not sure why that clear box is on top of it.

islandgrow

05-02-2008, 04:31 AM

found some more! can you see the foot! haha

shadowlord

05-02-2008, 05:05 AM

Very nice, professional, little set-up you have there. Thanks for the Pictures! I need to fabricate some new equipment as I plan on doing PFTek cakes this time rather than bulk substrate. You have given me some good ideas to use!

gojo

05-02-2008, 09:58 AM

LC is a liquid culture, its a nutritional water mixture to culture pure mycelium, used to inoculate spawn(grains and flour mix) its a quick way to colonize your spawn, instead of injecting spores you inject mycelium, knocking a few days off incubation.

Ah, I'm surprised they don't sell this, although I guess it wouldn't meet "scientific study" standard of US legality?

Im using agar as gelatinous medium in a nutrient mix poured into petri dishes, making sure to use proper sterile procedures. Its my first time playing with agar and petri's, they can be used for many purposes, such as isolating clean mycelial growth from a wild or contaminated spore print. or for isolating spore germination over mulispore germ. Agar work is not required at all, its considered more for advanced hobbiest.

Cool! :batman:

I've been wanting to play with petri dishes ever since I read how Breeder Steve developed the method of passing elite clone via. genes in a petri dish. I understood that you could use the petri dish to "grow" your own mother with the exact genetic makeup of the elite clone it came from. Steve was going to offer a crap load of elites (not just his breeds) but it was too illegal even when he was in Switzerland. I would love to see a thread of the process, guess they have it over on shroomery.org?

I really want to know how to use the petri for passing cannabis genetics. Steve once said it's pretty easy and the research is out there. That would be so excellent, no more cuts in the mail, just little ass, inconspicuous dishes.

Also agar isolates usually then get transfered to a LC or straight to spawn depending on prefrence.

So you use the agar/nutirent mix to grow the mycelial? From there you can spwan on horse poo (for example) or add it to the LC?

Ill take and post some pics once I construct my first tub, its basicly a rubbermaid with holes in side at semi specific spot covered or filled with your choice of filter material. It is designed for bulk substrates, as a bulk substrate fully and properly colonized will create its own environment, in terms of humidity, and as for c02 half of the holes in the chamber are at the same spot as the top of the substrate, the effect is c02 produced falls out of the holes as fresh air is brought in from the other half of the holes near the top of the chamber.

Ill post a few variations of making them and we'll all find out which performs the best :D

Nice! I'm REALLY looking froward to that! :popcorn:

Read, listen and watch Paul Stamets, he's a smart one.:old:

Did you know Paul makes the fungi extracts for "New Chapter" pro-biotic vitamins and supplements? I'm gonna try and pick some up tomorrow to try it out; looks good.

gojo

05-02-2008, 10:08 AM

I use stainless steel containers to put the brf + verm cake and poo combination into this sits on top of the perlite... you may want to resoak the perlite once or twice week to keep it fresh, you could use a bit of hydrogen peroxide too...

So you have a moist layer of perlite with a bit of HP and then you put the cake/poo mix directly on top of the perlite? With a lid for the container right?

So how did you re-hydrate the perlite? Just by misting the cake/poo mix the perilte will get wet? Or do you not cover all the perlite with the cake/poo mix?

gojo

05-02-2008, 10:12 AM

Nice pics IG, thanks! Thats the "Marta Tech"?

gojo

05-02-2008, 10:24 AM

Phew!!!

Ok, thanks to everyone so far! I'm gonna start with inoculating jars (10-20) with Paul Stamets's mix for P.cubensis:

(Thanks GS!, btw, what site did you find this on?) 5 Cups Vermiculite, 1.5 cups brown rice flour, .5 cup ground Rye grain, .35 cup Finch seed, 2.25 cup distilled water

Then I'm gonna spawn on horse poo in mono-tubs after we get a look at Springs tests, nice!

Now I just need to figure out what strains I going to order...everyone has peaked my interest in the many strains!

Thanks all! :D

dpn

05-02-2008, 07:10 PM

yes the cake inside the stainless steel container just sits on top, the more surface area exposed the more the damp perlite can raise the humidity...
i used an old net curtain to soak the perlite in, the bath or sink work good... i use a misty mate to keep things humid, dont directly spray the mushies though as stagnant water can cause the mushies to abort (start to form and then die) https://www.mistymate.com/shop/component/page,shop.product_details/flypage,shop.flypage/product_id,95/category_id,1/manufacturer_id,1/option,com_virtuemart/Itemid,26/

gojo

05-02-2008, 07:14 PM

yes the cake inside the stainless steel container just sits on top,

Ah, so you didn't mix the cake and poo? Just put the cakes on the perlite without breaking them up? That's the way I've seen it done before, good results.

Have you used that Misty Mate with foliar nutrients for cannabis? It looks good but does it get clogged when using nutrients?

thanks

dpn

05-02-2008, 09:10 PM

ive just used it for mushies but i think it would be ok if you used some kelp and cleaned it often :)

islandgrow

05-02-2008, 09:19 PM

Hey gojo, ya thats the martha tek but its modified quite a bit, me and another guy who is/was a member of shroomry came up with it first and we worked together to finalize it.

Also just general advice keep your jars high off the ground most germs are within the first foot of the ground.

dpn

05-02-2008, 10:10 PM

thanks for the pics IG could you list all the items you used please... im might make me one :)

islandgrow

05-02-2008, 11:51 PM

I really hope you do!!

Ok so your gonna need one of theses

http://www.mainlandmart.com/foggers.html

and MAKE SURE you get a buoy I have the single head one and it generated plenty of humidity. The buoy is key to keeping it floating in the bucket at the perfect hight.

I would get a 20 gallon tub that is not see through the more water you can hold the less often you have to fill it up. (for your fogger and buoy)

A tub that you are gonna grow them in

A fan that you can take front off (the picture above if you don't get what I'm saying)

hose to run it from the 20 gallon tub the tub that you are growing them in.

A submersible aquarium tank heater that you can adjust the temperature on.

A valve at the base of the hosing to control the flow of fog from one tub to the other.

They also sell on that website above a 3 stage mist controller that I bought and its helpful if you want to generate less fog but still have lots of fresh air exchange.

A glue gun to glue it all together

sometimes a timer is a good thing to have so you can control how often you are putting new fog inside it.

I think thats all, let me know if you have any questions.

oh ya, THINK TWICE CUT ONCE :)

Springs

05-02-2008, 11:54 PM

The Cannarchist

05-03-2008, 05:07 AM

So all you stoners are trippers as well.....hahaha.

I've lost more brain synapses than I could count so I save myself just for the herb these days.(Old fart I know...)

But if you want the maddest trip try the Thai mushies.....

Years of practice on Thai beaches....Mushie shakes for breakfast.

gojo

05-03-2008, 05:41 AM

Just wanted to say if anyones going to buy spores lets try not to get doubles between us, cause we can share and such, I can make up clean syringes no problem, of anything I grow.

peace,

Thats a great idea :up: ...though you'll have to teach me how to make a syringe, or maybe you've got a link?

Im gonna look through some of the banks tonight and make a list. I'll post it here and we can compair; I wont' get what is on the list you have posted.

:D

gojo

05-03-2008, 05:44 AM

But if you want the maddest trip try the Thai mushies.....

Nice, thanks for the tip. Im making my list tonight and I'll be sure to get a few :D

Years of practice on Thai beaches....Mushie shakes for breakfast.

Sounds good!

shadowlord

05-03-2008, 08:55 AM

just a quick check in, Ill read through and respond fully later on, busy simmering grains atm.

Just wanted to say if anyones going to buy spores lets try not to get doubles between us, cause we can share and such, I can make up clean syringes no problem, of anything I grow.

peace,

I'm getting a 10 pack of syringes, They are only like 10 bucks or something like that but I don't need 10. And I have several prints. If anybody is serious but doesn't have the scratch to buy, I will be happy to donate to get you started.
First come, first serve, limited time only LOL! This is a small site so hopefully I won't get 500 respondees Ha Ha HA. I have a COUPLE of prints not hundreds. But I am willing to share.
Peace.

Springs

05-03-2008, 12:11 PM

Ah, I'm surprised they don't sell this, although I guess it wouldn't meet "scientific study" standard of US legality?

Im sure some edible suppliers offer lc, no one offers LC for illegal mushrooms because mycelium is mushroom so its illegal, spore are ok because we are not growing them, purely microscopy intrest only.

LC's can be a bitch or a dream, the are not to be treated lightly, I jumped the gun and just lost alot of spawn due to bad LC's. Live and learn.

Dont try injecting spore to lc, get a pure mycelial sample and use a glovebox or flow hood to transfer, i did the opposite and lost. :P

Heres a really nice tek from Roger Rabbit at the shroomery:

For those who wish to make LC for inoculation, here's a way to get 100 syringes full of inoculant in two weeks: 1) Inoculate a quart jar of rye berries or wbs with your spores. Upon full colonization, shake the jar to loosen each individual kernel. Be sure to give it the smell test to make sure it smells like fresh mushrooms. 2) PC a quart of distilled water in a jar with a filter disk or tyvek, etc. In a glovebox or in front of a flowhood, pour 2/3 of the quart jar of sterilized water into your jar of shaken grains. 3) Shake well and then pour the now mycelium rich water back into whatever was left of your jar of sterilized water. Use the jar lid to hold back the grains themselves from pouring out. You now have a full quart of highly concentrated mycelium water that can then be used as is, or diluted two or three times again, and used to fill syringes. Because the grains were shaken first, the mycelium is ripped into shreds and will be sucked through even small needles easily, although larger needles always work better for this.

Remember, mycelium grows poorly in water unless under constant stir which oxygenates it. With grains, they colonize in two weeks or less because oxygen is throughout the jar in the spaces between the kernels.
RR

Cool! :batman:

I've been wanting to play with petri dishes ever since I read how Breeder Steve developed the method of passing elite clone via. genes in a petri dish. I understood that you could use the petri dish to "grow" your own mother with the exact genetic makeup of the elite clone it came from. Steve was going to offer a crap load of elites (not just his breeds) but it was too illegal even when he was in Switzerland. I would love to see a thread of the process, guess they have it over on shroomery.org?

I really want to know how to use the petri for passing cannabis genetics. Steve once said it's pretty easy and the research is out there. That would be so excellent, no more cuts in the mail, just little ass, inconspicuous dishes.

I think youre refering to micropropigation, I read his posts, i forget if he actually said he had success. I know chimera was also experimenting with it,
from what I understand its seems very feasable, theres tons of science type research in the subject focusing on other plants, so the info is available.
I dont think micropropigated tissues of cannabis would be any more illegal than not, but im not familar with the laws that crazy swiss may have :D

So you use the agar/nutirent mix to grow the mycelial? From there you can spwan on horse poo (for example) or add it to the LC?

Yep, i use agar to grow mycelium, even though thats not what always end up on the dish :)

From agar you can transfer to spawn substrate(grain, flour mix, woodchips), LC, or more agar. Agar to a bulk substrate(poo's, straw's(no hay!), logs, compost) is going to end in failure.

Did you know Paul makes the fungi extracts for "New Chapter" pro-biotic vitamins and supplements? I'm gonna try and pick some up tomorrow to try it out; looks good.

I did but have not tried any of them, im sure they will be great though, let us know what you get.

@shadowlord,
In the past i bought them at a feed store, im sure Ill find some, need some smaller ones for sharing, I prefer big syringes, harder to find but 180cc's is sure convenient. :P

What strains have you grown shadowlord?

I did some small reading the other night about the reishi, specificly the awsomeness and power of the reishi spores, alot of people say its the easiest to grow mushroom even more so than cube's. All the more reason to check it out!:woohoo:

Green Supreme

06-06-2008, 10:37 PM

:bump: Just wonderin how you funguys are doing. Peace GS

gojo

08-04-2008, 07:11 PM

hey all,

OK, well I'm gonna start this week. Gonna order some syninges this week and jars, etc.

So Im' gonna make cakes (PF tek) and then take the cakes and break them up into the top layer of bulk substrate within a mono tub.

So, my Q's:

Is there a good place to order bulk substrate from? I am thinking of making my own but I'd like to use a bulk substrate I know will work, at the first few times I use the mono tub.

Is the mono tub a high yielding method?

I don't remember too well, but does it take about a month or two from the time of injecting spores into jars to harvesting from mono tub?

After the first harvest in the mono tub they will re-pin right? Or do they just continually grow and continually harvest?

Anything I missed?

And for stuff I'll need:

pressure cooker

coffee bean grinder

any other expensive 'tools' I need?

I'll do a grow along with pics once I get everything in by next week :up:

Thanks! :)

gojo

08-04-2008, 07:25 PM

Nice!

I just bought the newish book:

"Psilocybin Mushroom Handbook: Easy Indoor and Outdoor Cultivation" at B&N for about 20.00!

http://www.erowid.org/library/books/psilocybin_mushroom_handbook.shtml
A thorough introduction to the cultivation of psychedelic mushrooms. Journey from one end of the mushroom life cycle to the other by the simplest and most direct route. Mycologists Nicholas and Ogame update and improve classic methods for germinating and growing the popular indoor species Psilocybe cubensis. They also explain how to grow Psilocybes outdoors using species that fruit on wood chips. the extensive color section lets you watch the mushrooms grow with photographs that meticulously record the processes. Each featured species is pictured to assist with identification.

Find essential information on:

The supplies you need, and where to get them
Mushroom biology and life cycles
"Sterile culture technique" practices
Updated PF Tek (Psilocybe Fanaticus technique) methods
Starting and growing on agar, grain, and wood chips
Making spore syringes and spore prints
Harvest and storage tips
Guidelines for preparation and use

With their straightforward instructions and proven techniques, you will be well on your way to a thriving garden of these beautiful, fascinating, and extraordinary organisms.

Anyone read this book before?

gojo

08-04-2008, 07:59 PM

OK,

I think I've got it figured out and have all the links, etc...

Here's a pic of a mono tub, thanks to it's creator "ohmatic". THe white stuff is called "poly fill".

Oh, yea, that book is geared for beginners and intermediate growers. It doesn't cover bulk substrate, etc, etc...

gojo

08-04-2008, 08:09 PM

OK,

Here's all the info I have, I'm doing mono tub from BRF cakes. I think these links will answer all Q's about mono tub...

The how-to mono tub tec thread by "ohmatic":
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5276282

Questions about making a Mono Tub (thread)
http://www.shroomery.org/forums/showflat.php/Number/5390649#5390649

Spawning in my Monotub (thread)
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5744361

A DIY inexpensive incubation heater, "introducing the 'heatbomb'":
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/3314778
(I am going to use this with a small igloo styrofoam cooler for BRF cakes, the mono tub doesn't need to be incubated, 75F is fine)

"Tennessee Stud" Bulk substrate for mono tub, put a 2-3" layer on bottom and crumble 1/2" layer of infected BRF cake onto surface and mix:
http://www.tennstud.com/page/page/1673026.htm
(go for the 50/50 mix)

ANd of course "RalphstersSpores" as the source:
http://www.ralphstersspores.com/

gojo

08-04-2008, 08:15 PM

Chilling?

Anyone chilled their cakes before moving into fruiting chamber?

This is how it goes, taken from a long post at shrommery:

Fully infected cake is washed off with distilled water and any pins are knocked off, the cake has nothing hanging off of it.

Then the cake is shaken dry and put in a plastic bag, air removed as much as possible and bag is sealed.

Bag goes into fridge for 24 hours

Take bag out and crumble cakes into nickel sized chunks.

(Optional?) Put nickel sized chunks into incubation chamber for 12 hours (80-85F?)

Take the cake chunks and lay over bulk substrate in fruiting chamber, ala mono tub.

gojo

08-04-2008, 08:33 PM

OK,

I bet chilling is used for the same reason as breaking up a fully infected sporebag:
Once your bag is 100% colonized it’s always a good idea to break the substrate up with your hands and let it re-colonize. This will inevitably make your mycelium network stronger.

gojo

08-04-2008, 08:41 PM

Oh ya,

GrowBags! I don't want to have to purchase a pressure cooker or glass pint jars. I'm gonna use growbags (ala sporebags):

inject 2-3 cc per bag and then put in incubation chamber at 80-85F until infected, 2-4 days.

Once fully infected I'm going to break it up and put back into incubation chamber for 2 more days. (I don't trust the chill tec just yet, haha)

Open sporebag and use that to lay on top of horse poo in mono tub to fruit.

I like sporebag as it's cheaper, pre-sterilized, has injection ports, less chance of newbie error (me lol), and much easier to infect large amounts of casings...I'm gonna blow it up so I don't' want fuc* around with little pint jars...

I'm thinking about using the The Mad Season:

http://www.themadseason.com/
Our Grow Bags, also known as MycoBags, contain our own grain based mix. The Grow Bags contain approximately 1 pound of substrate each. The bags themselves measure 18" H x 5.5" W x 4" D. They can be used to grow many types of mushrooms within the bag or can be used as spawn for a bulk substrate. They are pre-sterilized and include a self-healing injection site and a filter patch to allow for gas exchange. Mushrooms may be grown inside of the Grow Bag or you can take the colonized substrate out of the Grow Bag and case it with your favorite casing mix. All that is required is a spore syringe. Our Grow Bags are a great alternative to many of the other overpriced mushroom growing kits on the web today. Check them out to see why it doesn't get any easier than this!

The Mad Season growbag directions...too freaking easy!!!
http://www.themadseason.com/directions.html

:D

gojo

08-04-2008, 08:44 PM

Soooo....

With using growbags, DIY incubator with "heat bomb", Teen stud horse poo and DIY mono tub I think a $100-200 investment will produce A LOT of fungals! :)

Springs

08-10-2008, 08:29 AM

Hey gojo, sorry for the tart response, glad to see you are diving in, thats the most important part, is actually starting, practice makes perfecti.

Basicly Id say your plan is alright if you want to just try this out, there no way to tell what the results will be. The way I look at is if you aquire the base equipment to produce your own sterile spawn(a pc and jars) you are guaranteed success as you can hone your skills and learn from direct experience.

The bags from that site are one pound, lets be nice and say thats dry weight, its incredible expensive, for under $20 one can purchase 88lbs of grain. Empty bags can be purchased buy the 200 for under $100 which can be filled with 5 to 10lbs of spawn, pennies to the pound.

Monotubs, I dont like them, read RR's comments, its more than neglectfully its grossly so, I mean once at the fruiting stage contams are not a major concern, the less fresh air the more chance of contams, I would of had complete failure of my mono's if I had kept it truely "mono" and left the lid on, fan and mist works like a charm. That being said I dont like rubbermaids as much as I use to, im going towards a martha style, shelves, with automated rh/fae, with bins of cased sub.

Time from inoc to harvest varries greatly, but usually within 2months, optimal 1 month. Many paramaters.

Pinning is much the same many varriables, multi spore will is more prone to be patchy sparatic pinset depending on how multi it is.

Read Stamets for details on all of this, his cult book is on the shroomery, its a must read.

Everyones situation and goals are different, keep at it.

Oh and ralphsters is great, dont spend unneededly though, Ill have most of his collection sooner than later.

All the vendors you listed are shroomery sponsers, thus trustable, so dont worry about that.

Keeps us in the loop, like you do so well with your other cool projects!

Peace.

gojo

08-10-2008, 09:30 PM

Hey sprigs,

:)

Nice pics!

So in your quasi-mono tub you have a lot of holes with poly, not just two or three. And why the dark plastic? I thought you wanted light to reach the fungals? I know theres some debate on that but it makes sense to me as they would have light in Nature. I thought your supposed to use a clear container and then wrap a light-blocking material around the base substrate in the tub?

What is the tube sticking into the tub?

Basicly Id say your plan is alright if you want to just try this out, there no way to tell what the results will be. The way I look at is if you aquire the base equipment to produce your own sterile spawn(a pc and jars) you are guaranteed success as you can hone your skills and learn from direct experience.

The main reason I'm going to inoc into the bags is to save some money on the jars right now. In the future I'll use jars but that means I need to buy a pressure cooker, etc, etc,etc. I just don't have the extra money right now for the scale I'm going to do. I am going to blow it up!

As for results, the spawn bags should work well (I hope, lol) as I'll spawn into them and then use it in the mono tub.

Oh, and I'm going to make LC and other neat things later on...with your assistance if you don't mind :)

The bags from that site are one pound, lets be nice and say thats dry weight, its incredible expensive, for under $20 one can purchase 88lbs of grain. Empty bags can be purchased buy the 200 for under $100 which can be filled with 5 to 10lbs of spawn, pennies to the pound.

That bag is pre-moistend and ready to go. But I totally agree with what you wrote. And I want to do everything myself, once I have the extra money for the pressure cooker, jars, etc.

By using what I've described my initial $$$ input is low vs the big yield I can achieve. After this first pin and harvest I'll do it right...

Monotubs, I dont like them, read RR's comments, its more than neglectfully its grossly so, I mean once at the fruiting stage contams are not a major concern, the less fresh air the more chance of contams, I would of had complete failure of my mono's if I had kept it truely "mono" and left the lid on, fan and mist works like a charm. That being said I dont like rubbermaids as much as I use to, im going towards a martha style, shelves, with automated rh/fae, with bins of cased sub.

Yes, I agree here to. I am using mono tubs as they are the least expensive for me right now. And I plan to mist with distilled water a few times a day, when I mist the air will be exchanged, that sounds accurate right?

I will look more into the martha tec, I will re-read this thread again. And I'll search on the shrommery for how-to threads, if I find good links I'll post them here :)

And I was under the impression that less air exchange offered less chance of contamination? ANd like you said, and I've read, once the inoc is complete (from a jar or spawn bag) the chance of contamination is much lower, that's why it's ok to 'dunk' the cakes.

Pinning is much the same many varriables, multi spore will is more prone to be patchy sparatic pinset depending on how multi it is.

By multi-spore do mean inocing with a thai and golden teacher in the same jar?

Read Stamets for details on all of this, his cult book is on the shroomery, its a must read.

Will do! Have you read "Psilocybin Mushroom Handbook: Easy Indoor and Outdoor Cultivation"?

Oh and ralphsters is great, dont spend unneededly though, Ill have most of his collection sooner than later.

What don't you have? I will order some of those and then we can trade. For now the most important factors in my choice of stain/s is:

Yield

Potency

User-friendliness (a strain that can take some grower errors ;) )

And last would be speed of pin and fruit.

Thanks!

Springs

08-11-2008, 02:53 AM

Hey sprigs,

:)

Nice pics!

Thanks :)

So in your quasi-mono tub you have a lot of holes with poly, not just two or three.
Ive done many variations, I like no holes, keeps the rubbermaid useable.
And why the dark plastic? I thought you wanted light to reach the fungals? I know theres some debate on that but it makes sense to me as they would have light in Nature. I thought your supposed to use a clear container and then wrap a light-blocking material around the base substrate in the tub?

I have a large rectangle cut into the lid, I have a piece of poly with slits inbetween the lid and bin. So the poly is the actiual cover, and the lid is used to hold the poly in place, I have a light shine through the lid. I wouldnt say there is a debate about light, mushrooms can grow with no light, but that is not to say they will grow healthy, or "normal" Even though it appears dark the maids still let light through and I still get side pinning.

What is the tube sticking into the tub?

I thought you'd spot that. I have a coolmist blowing into a garbage bag which has tubing running to maids, basicly automated rh/fae, although I still mist and fan manual, gotta say hi a few times a day :)

The main reason I'm going to inoc into the bags is to save some money on the jars right now. In the future I'll use jars but that means I need to buy a pressure cooker, etc, etc,etc. I just don't have the extra money right now for the scale I'm going to do. I am going to blow it up!

Good stuff, just keep at it and it will eventually snowball. I suggest reading all of agars posts, he's thoroughly documented how to blow up waste items into mushrooms.

As for results, the spawn bags should work well (I hope, lol) as I'll spawn into them and then use it in the mono tub.

You mean you'll innoculate into them, then spawn run into hpoo, which you'll place into a monotub as a fruiting chamber :) gotta get the lingo down :p, you could "spawn into a bag" with grain to grain, or grain to bulk in a bag.
Either way im sure it'll work just fine, if not try again no biggie.

Oh, and I'm going to make LC and other neat things later on...with your assistance if you don't mind :)

No problemo's.

That bag is pre-moistend and ready to go. But I totally agree with what you wrote. And I want to do everything myself, once I have the extra money for the pressure cooker, jars, etc.

By using what I've described my initial $$$ input is low vs the big yield I can achieve. After this first pin and harvest I'll do it right...

Yes, I agree here to. I am using mono tubs as they are the least expensive for me right now. And I plan to mist with distilled water a few times a day, when I mist the air will be exchanged, that sounds accurate right?

Sounds good, it would depend on how the tub looks at the moment if its to humid I would give a few fans with the lid with a light mist or none, or if its looking to dry the opposite.

I will look more into the martha tec, I will re-read this thread again. And I'll search on the shrommery for how-to threads, if I find good links I'll post them here :)

yeah, lots of good stuff at the shroomery, check out the no pressure cooker grain post thats a recent thread in the advanced cult forum.

dont forget stamets, its more important than a pressure cooker, thankfully one can read it for free. Take advantage of that and read it over and over again.

And I was under the impression that less air exchange offered less chance of contamination? ANd like you said, and I've read, once the inoc is complete (from a jar or spawn bag) the chance of contamination is much lower, that's why it's ok to 'dunk' the cakes.

yep common misimpression, hard for something "bad" to get a foot hold when the co2 is low, which is what most pathogens(and non) like to incubate in, basicly its the opposite of incubating, where you want elevated co2 thus the danger of contams, they like it too. Different mushrooms have differnt co2 paramaters, the oyster mushroom is very sensitive to co2 during fruiting, and will produce very poor mushrooms if there is not enough fae.

By multi-spore do mean inocing with a thai and golden teacher in the same jar?

Nope when two spore get together and perform there sacred ritual of sporulatic love they produce a unique perfecti mycelium, if you inject your substrate with more than 2 spores you will end up with more than one mycelial growth(for lack of scientific term, which id know if read stamets as much as i should :P) so like the pic of my tub from the top down, that tub has been flushing ying/yang style, one side is starting as the other is ending and vise versa, this is do to the multi spore innoculation procedure.

Which leads to cloning or isolating, which can be done with agar easily or also direct to grain. Basicly cloning a mushroom is taking a small piece of the mushroom your cloning and tranfer that piece to a sterile medium, agar or grain usually, while making sure the piece is also sterile. Or before you produce a mushroom you can germinate the spore on dish's with a agar or agar like nutritive medium again sterilized, to let the mycelium grow and then you can cut out sectors of "pure" growth.

Will do! Have you read "Psilocybin Mushroom Handbook: Easy Indoor and Outdoor Cultivation"?

Who's the author?
Probably not though, ive taken advantage of the free info for the most part.
Its probably solid though.

What don't you have? I will order some of those and then we can trade. For now the most important factors in my choice of stain/s is:

Yield

Potency

User-friendliness (a strain that can take some grower errors ;) )

And last would be speed of pin and fruit.

Thanks!

gojo

08-11-2008, 11:43 AM

gojo

08-11-2008, 11:49 AM

Oh,

And that book I was talking about was written by:

L.G. Nicholas and Kerry Ogame

Here's a link to info for it:
http://www.erowid.org/library/books/psilocybin_mushroom_handbook.shtml

thanks :D

gojo

08-11-2008, 12:19 PM

and one more thing,

Is "casing" the right term for the horse poo and shredded staw mixutre? I don't think casing is the right word but I'm not sure.

thanks

gojo

08-11-2008, 12:40 PM

Hey,

Neat. Ralphsters sells their own bulk substrate (I think that the right term, not "casing", correct?).

But man it's expensive!!! A pound for $18-20.00 vs a pound of Tenn Stud horse poo/straw mix for $3.00 a pound. Though I like the sound of the zoo mix. I think I will get 2 pounds of the "treated zoo mix" from ralphsters and 20-30 pounds of Tenn stud poo/straw mix.

I think I will do a little side by side grow: the zoo mix vs the Tenn stud mix. And I want to mix some zoo into the stud, maybe at like 2-5%? Thoughts?

http://www.ralphstersspores.com/index_files/Page7171.htm
Although the bulk of our spores are for Microscopy use only, we still get many requests from customers for the best substrate to use for growing their favorite edibles.

In response to the many requests… We are now offering the absolute best and easiest substrate available. All are specially treated with Ralphsters exclusive formula and come packaged in a reusable 6 x 10” cloth bag [approx 1 lb dry; I wonder what it expands to???], just drop the bag into a pot to pasteurize, let it drain by hanging by it’s drawstring, let it cool and your ready to add your favorite edible spawn.

Treated straw.
$16.00

Treated straw and horse dung
$18.00

Treated straw and a zoo mix
$18.00

Treated horse dung.
$20.00

Treated zoo mix.
$20.00

Zoo Mix is elephant, zebra, rhino and giraffe, dung.!!

gojo

08-11-2008, 01:47 PM

Paint???

Anyone know of a paint which would protect my closet walls (made of dry-wall) from the high humidity? Maybe a latex base coat type of paint or something? And isn't there some kind of mold resistant type of paint or something?

Thanks :)

c-ray

08-11-2008, 01:52 PM

kitchen and bathroom paint, just ask at any paint place

Green Supreme

08-11-2008, 07:57 PM

I used marine paint in my kitchen once. Very durable but maybe a little overboard. Peace GS

Springs

08-12-2008, 04:53 AM

Thanks Springs!

Got you PM :) I'll reply soon.

I looked at that thread at the shroomery about no pressure cooker. neat. They are fermenting to grow LAB which out compete harmful microbes and some fungals feed upon the LAB. I have not read the whole thread yet but does p.cubensis feed upon LAB? Even if not I bet that would work fine as LAB have long relationships to many fungals. Oh, you may want to check out C-rays experiment fermenting his own rejuvalac(sp?) to culture LAB and yeast, though the yeast can be scooped out like in that thread. Crays' post in the last page of the BIM thread in the Organics sub-forum.

I am definitely going to try using LAB without a pressure cooker but it takes longer. Right now I can't afford to experiment but I will soon!

Ill know very shortly if it works with cubes, yep its just like making rejuvilac, ive yet to taste it, im optimistic though. I guess Im just used to that smell being a bad thing when it comes to this hobby, let alone now consuming the source of said smell. Either way its a very exciting path of cultivation that much good will come from.

Do you have any links to the Stamets info your talking about?

I failed with a quick search of the shroomery, torrent sites have it, usually in a big package of stamets material. If you still cant find anything, pm me ill hook you up.

Ok, so after re-reading this thread and the info you've posted I've changed my mind and I'm making a martha-like tec but I'll be using my closet instead of the Martha Stewart storage tent.

My Martha-Like Fruiting Closet:

Temp: 75-80F

Humidity: 85-90

Size of my closet: 2.5 feet deep x 4 feet wide x 8 feet high

I will tape clear polly over the whole doorway opening to my closet. And I will use a "Tarp Zip-Up" to add a doorway for $10.00. I will tape the door to the polly. I am also going to cut a few 1/8" holes about 2' high from the floor in the polly. I will fill the holes with polly-fill and glue it in place, I think this should help the Co2 escape as the humidifiers run providing humidity and all the FAE that should be needed.
Tarp Zip-Up: http://www.discount-hydro.com/productdisp.php?pid=319&navid=33

Im not sure the zipper is necessary, if you have cool mists pumping in there will be enough positive pressure that if you just draped poly overlaping the entrance poly, you could also use dollar store magnets to attach a poly door, I wouldnt worry about sealing the door way so much though.

I will clean the shit out of the closet with a 10% bleach solution in water to wash walls, etc.

I will stack racks in the closet, one rack every 9-10 inches, starting at the 2' mark and going all the way up to the 6' mark.

I will put [2-3] four foot, two tube, florescent lights hung vertically in front of the trays but on the outside of the plastic door.

Sounds good, I think 2 should suffice, how many watt's?

Then I will spray a good bit of Lysol to disinfect the closet and zip up the door and let it marinate for a few days. (Is the Lysol required?)

If you have iso spray iso, if not I wouldnt spray lysol type sprays in a f/c, if I did I would wipe it off after a few minutes. Lysol is not required but can help, I suggest the Clorox brand disinfectant spray its always been the cheapest with more product, same chemicals. My latest bottle of lysol was $5 Ive bought clorox for $2.50

I will cover each tray with aluminum baking pans filled with colonized substrate and casing substrate (horse poo and shredded straw mixture)

I will buy 2 cool mist humidifiers and add a hose from them ducting into the polly and into the fruiting closet (ala Martha tec). I will run them 24/7 or 45 min ON and 15 min OFF, depending upon humidity levels.

If the humidifiers don't provide high enough humidity I'll use a ultrasonic humidifier. If I need it I'll use it I will run it for 2 min ON every 2-3 hours.
[/LIST]

My Plan:
(please let me know if you see problems)

Inoculate into spawn bags in a DIY heat bomb incubation chamber at 82-86F

If moneys tight and you dont have a fish tank heater just use a light bulb with the light shielded away, I dont use any external heating, too much heat is worse than not enough. If you can get it close to 80 your laughin.

After colonization I am thinking about putting the bags into a pot filled with cold distilled water and place that into the fridge for 24 hours (ala "dunking"). (What do you think?)

It may help, ive cold shocked pf cakes way back, some say it help others say it makes verry little difference. Its adapted from gourmet cultivation of specific species which require the temps to dip, like in fall, to fruit. Cubies are tropic and pan tropic, they dont need the cold shock. For a quick flush from incubation a fully colonized substrate is more important.

Then I will mix the colonized substrate with horse poo/straw (50/50) casing in aluminum baking pan (cheap kind) to top of pan

Mixing colonized substrate and casing:

1/2-1" bottom layer of casing

1/2-3/4" middle layer of colonized substrate (spawn)

Final top layer of casing (1/4 of the depth of the middle substrate layer)

I will keep the lights off while casing is being colonized for a few days, what temp should it be? I think 75F right? (when should I turn the light on?)

Once pining starts I'll use the fluros on 15 hours

A while later full fruit should be happening :)

Sounds good, switch things up though to see what will work best, try some with out a bottom casing, varry the depth of the casing's and subs. I wouldnt fruit with more than 12 hours.

Links I found useful:

"From syringe to print using rye": A complete how-to from start to finish with rye in jars with martha tec. See "The Chamber" section for martha info.
http://www.shroomery.org/8432/From-syringe-to-print-using-rye

And here is the same how-to but has missing info, this however is the whole thread with 8 pages:
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/3039921/page/0/fpart/1/vc/1/nt/8

"Cybers Martha Tek": Great step-by-step for building a martha tec style fruiting chamber.
http://www.shroomery.org/9664/Cybers-Martha-Tek

"A Report w/ pics and links--FAE, RH, Casings, Cakes, Polyfil, And A Simple Setup And More!":
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6670105

Thanks!!!

Check out:
Magash's clone and fruit: http://www.shroomery.org/forums/showflat.php/Number/6615452#6615452

Magash's martha: search magash's post with the keyword martha, not a closet but lots of good things that can be adapted.

scatmanmarv's closet conversion guide: http://www.shroomery.org/forums/showflat.php/Number/4024838#4024838

guide of goodness: http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8467229/page/0/fpart/1/vc/1/nt/2

Oh,

And that book I was talking about was written by:

L.G. Nicholas and Kerry Ogame

Here's a link to info for it:
http://www.erowid.org/library/books/...handbook.shtml

thanks

Cool, how do you like it?

and one more thing,

Is "casing" the right term for the horse poo and shredded staw mixutre? I don't think casing is the right word but I'm not sure.

thanks

nope the correct term would be your bulk substrate mix, casing is the protective non nutritive layer compromised usually of a peat mix, or a coco coir mix.

Hey,

Neat. Ralphsters sells their own bulk substrate (I think that the right term, not "casing", correct?).

But man it's expensive!!! A pound for $18-20.00 vs a pound of Tenn Stud horse poo/straw mix for $3.00 a pound. Though I like the sound of the zoo mix. I think I will get 2 pounds of the "treated zoo mix" from ralphsters and 20-30 pounds of Tenn stud poo/straw mix.

I think I will do a little side by side grow: the zoo mix vs the Tenn stud mix. And I want to mix some zoo into the stud, maybe at like 2-5%? Thoughts?

http://www.ralphstersspores.com/inde...s/Page7171.htm
Quote:
Although the bulk of our spores are for Microscopy use only, we still get many requests from customers for the best substrate to use for growing their favorite edibles.

In response to the many requests… We are now offering the absolute best and easiest substrate available. All are specially treated with Ralphsters exclusive formula and come packaged in a reusable 6 x 10” cloth bag [approx 1 lb dry; I wonder what it expands to???], just drop the bag into a pot to pasteurize, let it drain by hanging by it’s drawstring, let it cool and your ready to add your favorite edible spawn.

Treated straw.
$16.00

Treated straw and horse dung
$18.00

Treated straw and a zoo mix
$18.00

Treated horse dung.
$20.00

Treated zoo mix.
$20.00

Zoo Mix is elephant, zebra, rhino and giraffe, dung.!!

Yeah Id go with your wallet on this one, the best supplement is spawning at a higher ratio, or using baked grain. Industrial farm supplements are seeds.
Id spend the $20 on a few bales of straw and youll have enough material for a year probably. You can ammend the straw with all sorts of stuff you have.

Use worm castings aswell! They make a great sub.

Peace

gojo

08-12-2008, 07:47 AM

Hey C-ray and GS,

Thanks for the heads up! I'll go check it out. The marine paint sounds worth a look too, I'm a bit worried about the 90+ humidity.

thanks :)

gojo

08-12-2008, 08:13 AM

Hey Springs,

Thanks for the info and links, I'm reading them tomorrow.

I failed with a quick search of the shroomery, torrent sites have it, usually in a big package of stamets material. If you still cant find anything, pm me ill hook you up.

Yea I found a lot of stuff, over 850 mb !!!

Sounds good, I think 2 should suffice, how many watt's?

80 watt per light (40 watt per bulb)

If you have iso spray iso,

What is iso?

I don't use any external heating, too much heat is worse than not enough. If you can get it close to 80 your laughin.

So you dont' use a heater in your inoc chamber? And what do you mean by "laughin", is that good? Do you mean I should try for 80 instead of 82-86?

Cool, how do you like it?

I like it well so far but I've been doing more reading at the shrommery then the book.

nope the correct term would be your bulk substrate mix, casing is the protective non nutritive layer compromised usually of a peat mix, or a coco coir mix.

I can just mix my inoc substrate into the bulk substrate and I don't need a casing layer right?

Use worm castings aswell! They make a great sub.

Yea I was thinking about that. I have a ton of EWC and I can get straw. And I've read threads at the shommery showing how to pasturize the mix, ala horse poo.

thanks!

Lungus

08-12-2008, 04:13 PM

What is iso?

Isopropyl alcohol.

Springs

08-12-2008, 07:35 PM

Hey Springs,

Thanks for the info and links, I'm reading them tomorrow.

Yea I found a lot of stuff, over 850 mb !!!

80 watt per light (40 watt per bulb)

You could probably use one, if you want to save the $kwh. Im using a 12w cfl, and try to move it around once or twice a day, tubs are in room with full ambient sunlight aswell.

What is iso?

Lungus nailed it, I prefer 99% if you can get it, others like 70%, some even mix the two to make 80%, I find it cheap at commercial cleaning supply stores.

So you dont' use a heater in your inoc chamber? And what do you mean by "laughin", is that good? Do you mean I should try for 80 instead of 82-86?

Laughing is always good :) I just meant that the difference between 79 and 85 F is very little, I would go with ghetto tek to save money if need be.

I like it well so far but I've been doing more reading at the shrommery then the book.

Yeah ive spent countless hours reading threads, its a good way to learn.

I can just mix my inoc substrate into the bulk substrate and I don't need a casing layer right?

Dont need, but would probably be better to use than not, its another step yes, but its better to get used to it now and get some experience, its cheap and less labor than pasturizing or sterilizing other materials, you can even use a microwave.

Yea I was thinking about that. I have a ton of EWC and I can get straw. And I've read threads at the shommery showing how to pasturize the mix, ala horse poo.

thanks!

:good:

Then your gold man, mix your spawn, ewc, and straw at different % untill you find optimal results.

Also you do not need a pressure cooker for the PF tek style of sterilization, basicly when using a flour and verm mix, you also dont need to use jars, you can use #5 recyclable plastics, like microwaveable ziplock containers, which you then steam sterilize.

peace

gojo

08-12-2008, 08:41 PM

Dont need, but would probably be better to use than not, its another step yes, but its better to get used to it now and get some experience, its cheap and less labor than pasturizing or sterilizing other materials, you can even use a microwave.

So I would:
inoc the jars (or spawn bag) and once it's colonized I would mix the spawn with a casing layer (ex. from "From syringe to print using rye") and then put it into the incubator and let the casing get fully colonized. Then I would mix the casing with the bulk substrate in my f/c? (yep, getting the lingo down, lol ;) ) OR, would I mix the spawn with the bulk substrate and then put that into the incubator and let it get coloznied. Then mix that with the casing layer in the f/c? (That's how Tenn Stud does it)

Also you do not need a pressure cooker for the PF tek style of sterilization, basicly when using a flour and verm mix, you also dont need to use jars, you can use #5 recyclable plastics, like microwaveable ziplock containers, which you then steam sterilize.

Would you mind describing this process a little or do you have any links? I would rather use this method as I already have everything. But I'm a bit worried I'll fuc*up and ruin the whole run...and I can't afford that. I assume you mean I just drop the ziplock bags into hot water or into the microwave?

I like the idea of using ziplock bags and not jars. After they cool down I guess I could wipe them down with alcohol wipes. Then I could put a small piece of Tyvuk(sp?) tape on the outside of the bag and inject through it? Would it be wise to put a layer of alcohol wipe over the Tyvuk tape and then cover the alcohol wipe with another piece of larger Tyvuk tape?

Or should I just order those fancy spawn bags? How do they seal up? A light vacuum seal?

And about the BRF/Verm mix, does that provide 'good' nutrients for the inoc? I was thinking about the Stamets mix GS posted, but that would prolly need a pressure cooker right?

:D

Thanks man. Sorry for all the Q's and thanks for all the help! I'm getting this week Ecuador and Hawaiian.

gojo

08-12-2008, 08:50 PM

Hey all,

Here is a nice horse poo tec by RoadKill for making your own horse poo bulk substrate at home. I am going do this but also I am going to use a 50/50 mix of EWC and shredded straw in place of the horse poo. Springs, I can do that right? Just replace the instances of horse poo below with EWC and straw (50/50 mix)?

This is how Tennessee Stud makes their bulk substrate (ala "RoadKill Horsepoo Tec") and it's very popular.
http://www.tennstud.com/page/page/1678240.htm

The following is a brief introduction to using Tennessee Stud Mushroom Substrate. If you want to learn more about growing mushrooms, please visit the link provided at the bottom of the page

Make sure you work in a clean environment. As a rule of thumb, a 1:10 spawn to substrate ratio is optimum for innoculation.

Always wash your hands before working with substrates & spawn. Open your Pre-Pasteurized substrate bag and pour out 1/4 to 1/3 into the bottom of a plastic container (The amount depends on th size of the container). Rubbermaid type dish pans or inexpensive plastic kitty litter containers work well. It really doesn't matter what type of container you use, just make sure you can add 3-6 inches of substrate and leave at least 2-3 inches below the top rim. Also make sure that light cannot get through the container. If you are unsure if any light can get through you can tape the sides and bottom with black electrical tape, foil, duct tape or something that will block any light from getting through. This will prevent mushrooms from growing below the surface.

After you pour 1/3 to 1/4 of the substrate into the container add a thin layer of spawn to the top. Mix this together in the pan and repeat the process until you get to the recommened level. We also recommend that you sprinkle the top layer with an even layer of spawn. This will colonize quite quickly and help prevent contaminants from getting in.

Once this is accomplished cover the container with a garbage bag or foil. Poke holes in the foil and place another piece of foil to gently sit on top. This will allow some air exchange. If you use a garbage bag just cover it up and let the ends drape over the container so some air exchange is possible.

Place the container in an environment where it will maintain a temperature of 82-86F. In about 7-10 days your substrate should be fully colonized with healthy mycelium and is ready to be cased.

If you have any questions about using bulk subsrtrates I highly recommend this website. It's the best on the internet; Exlore it, Study it and Interact with it. If you are serious about this hobby, use this website to discover a wealth of information on growing all kinds of mushrooms.

Roadkill's Horse Poo Tek For Dry Substrate Preparation

Ok a hell of alot of people have asked for my horse poo tek. first off It's not my horse poo tek. It was taught to pleezr and I by Pavlov's Dog.....in person at his house. I have changed it a bit....but credit is due to him, not me....I'm no theif.

Also the using the food processor part was taken from OldTimer....I believe.

So here is the horse poo tek, Pav said that I could write this up!

You want the horse poo to be dried out....this is important.

Then you put some in a pillow case....amount depends on the size of your pot.

Tie off the pillow case.....Put it in a big ass pot.

put purified or mineral water in the pot....tap water is fine if you have good quality tap water.

Get a weight to hold the pillow case down....I use a 20 pound weight lifting weight.

get a candy thermometer.....you'll need one....most grocery stores have it.

turn the stove on and get it up to 160 to 175 degrees.

make sure by checking the candy thermometer.

this is called pasturizing. ....don't go over 175 degrees.

do this for 30 to 40 minutes.

after you are done.....let it cool down.

then ring the pillow case out into the pot.

after some good ringing out.....Put an oven rack over over the sink and place pillow case on top to drain.

leave in there for a hour or so...longer is better.

Keep the poo water....you can use that for your water mixtures....for like cakes or grains.....the added nutrients of the poo water is good!

I fill up empty gallon water jugs with poo water....Roses like it too.

Now that the pillow case is done draining after awhile....open it up and check it.

It should be moist.....not runny.....hand squeeze it out if you have to.

you can wash your hands later if you don't like icky stuff....doesn't bother me.

Get some standard size dish tubs(black colored prefered)

Fill the tub with 3" to 4" of the poo.

make sure that the poo is cool...after it is cool.....proceed.

Take a PF cake and put it in a food processor...and hit the start button a few times.

break the cake up into little peices....don't over do it...or you will get putty.

*** You will get thousands of inoculation points to the poo by using a food processor....cause the substrate will be well broken up and spread out more ***

If you don't have a food processor.....break the cake up by hand.

but if you break them up by hand...make sure that they are broken up really small.

You can spawn the cake into the poo that is in the dish tub.....mix it up really good....only a 1/2 pint cake is needed per pan.

If you want.....you can spawn with Grain, Birseed....etc.

Doesn't have to be a BRF cake.

Cover up the pan with Alum foil after you have mixed in the substrate with the poo.

Take a fork and punch little holes in several places.....like 9 places in the Alum foil though out the top.

Stick this in the incubator......and you should see growth in a day or 2.

After you get the whole pan of poo colonized you can case it with a Coco Coir casing or any casing layer you want.....or you can let it fruit in the fruiting chamber without a casing layer..... a casing layer can be better!

Of course you would leave the container in the incubator if you put a top casing layer on.

I would like to sincerely thank Roadkill for the above basic outline of Dry Substrate preparation. The man is my inspiration for this website.

This Tek can be used to pasteurize poo, straw or both

Just a couple of notes--Instead of pillow cases you can use 5 gallon nylon paint filter bags available at Lowes or paint stores like Sherwin Williams. They are re-usable and cheaper than replacing pillow cases. Whatever you use--put something on the bottom of the pot to prevent possible burning or melting of the bag. I like small round cake racks you can find for a buck at most dollar stores.

I recommend a metal cheese grater as opposed to a food processor for breaking up cakes. IMO it's too easy to turn the cake into mush using the food processor. Plus the grater is easier to wipe down with alcohol(allow to dry completely)prior to grating.

Springs

08-12-2008, 11:55 PM

So I would:
inoc the jars (or spawn bag) and once it's colonized I would mix the spawn with a casing layer (ex. from "From syringe to print using rye") and then put it into the incubator and let the casing get fully colonized. Then I would mix the casing with the bulk substrate in my f/c? (yep, getting the lingo down, lol ;) ) OR, would I mix the spawn with the bulk substrate and then put that into the incubator and let it get coloznied. Then mix that with the casing layer in the f/c? (That's how Tenn Stud does it)

The second one is closer, you are right to mix the spawn to the bulk substrate and allow it to colonize, once colonized you have a solid mass of mycelium, which you put a thin layer of casing over top of, no mixing with anything. When you get to this point im sure you will have done alot more research into it.

Would you mind describing this process a little or do you have any links? I would rather use this method as I already have everything. But I'm a bit worried I'll fuc*up and ruin the whole run...and I can't afford that. I assume you mean I just drop the ziplock bags into hot water or into the microwave?

I like the idea of using ziplock bags and not jars. After they cool down I guess I could wipe them down with alcohol wipes. Then I could put a small piece of Tyvuk(sp?) tape on the outside of the bag and inject through it? Would it be wise to put a layer of alcohol wipe over the Tyvuk tape and then cover the alcohol wipe with another piece of larger Tyvuk tape?

Or should I just order those fancy spawn bags? How do they seal up? A light vacuum seal?

Just search for PF tek, if youre not getting a pc and dont want to mail order sterile subs, then pf is for you.

The zip lock baggies wont work, the microwavable containers that zip lock makes with the blue lids. Not submerged in water but steamed. Shroomery is the place for this info, but a dozen half quart wide mouth jars are like 6 dollars, and can also be found at second hand stores, and thrift stores, where you can find humidifiers as well. Many people find great deals on cheap pressure cookers aswell. Check out garage sales aswell.

And about the BRF/Verm mix, does that provide 'good' nutrients for the inoc? I was thinking about the Stamets mix GS posted, but that would prolly need a pressure cooker right?

The nutrients are good for the mycelium, you can use many flours, mix's recomended! If the recipe gs posted doesnt have any whole seeds, and is a flour recipe which i think it was then you can get away with steaming without a pc. Ive only made pfcakes once and with a pc, which i crumbled and cased and placed in a perlite humidity chamber, worked awsome.

:D

Thanks man. Sorry for all the Q's and thanks for all the help! I'm getting this week Ecuador and Hawaiian.

Dont be sorry for questions, thats the whole point, its no problem, after all the info you've contributed the least i can do is answer a few mushroom cult questions ;)

peace

gojo

08-13-2008, 08:28 PM

OK,

You've talked me into just getting a PC and doing it right. Those spawn bags would take way too long to colonize and I found an inexpensive pressure cooker, I can use a cooker right? I don't need a canner?

This should be the way I'm gonna do it...I hope ;)

Make a Glove Box for sterile inoc (spray the inside with iso spray and pre-flame the needle then warp in iso soaked bandage cloth for transport into GB)
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/4992739/an/0/page/0

Making the lid for the injection with tyvek and micro-pore tape and finally a last layer of alc dipped micro-pore tape (after injection).
http://www.shroomery.org/forums/showflat.php/Number/6381868#6381868

Spawn: I'm going to use 1/2 organic rye and 1/2 finch seed. I'll soak for 24 hours and rinse, rinse, rinse! (Does that mix sound good? Should I add vermiculite also?)
http://www.shroomery.org/8432/From-syringe-to-print-using-rye

Make a few TiTs for jars to colonize, 80-86F
http://www.shroomery.org/8432/From-syringe-to-print-using-rye

After colorization of jars (20-25 quart jars) I'll mix up with two diff bulk substrates (Tenn Stud/staw and my own EWC/staw using RoadKill's How-to) at 1:4 (spawn/bulk) and put back into TiT for a few days.
http://www.shroomery.org/8432/From-syringe-to-print-using-rye

Once colonized I'll take them out and case with a mix of coir and vermiculite and put in the F/C (85-90 humidity and 70-80F)
http://www.shroomery.org/8432/From-syringe-to-print-using-rye

Once I'm ready to harvest I will try to make some LC or spore prints. What do you think about the newish(?) tec where you put the needle into the stem and suck out the 'stuff' to make LC?
http://www.sporeworks.com/syringetek.html

Does that sound about right?

P.S. Next run I'll prolly make my own bulk substrate and not order it, but well see...I'm curious to see if the EWC preform better then the horse poo.

gojo

08-13-2008, 08:33 PM

Oh,

what is the best yielder in your opinion? Another big factor for me is a low aborting strain, that's why I like the Ecuador and Hawaiian. I think I may order two syringes of the Hawaiian as it seems to have all I'm looking for: few aborts, strong, good yield, etc.

Thoughts?

Golden Teacher

Ecuador

Hawaiian (i know it's not a cube, but a PES right? Supposed to be way strong?)

gojo

08-13-2008, 09:04 PM

Hey all,

A bit more strain info...

For me (a newbie) I want a low aborter and good 1st flush. So I think I'll go with just Ecuador this run and next time some thai and hawaiian.

Good link I found useful:
What strain of Cubensis has the highest yield? (http://www.shroomery.org/forums/showflat.php/Cat/0/Number/584314/page/0/fpart/1/vc/1/nt/2)

Free Spore Ring (http://www.geocities.com/freesporering2002/) (anyone used this or know "ralphster444"?)

Psilocybe cubensis Ecuador

Cultivation Difficulty: Easy
Potency: Moderate to Potent
Substrate: Dung, Straw, Various grains
Colonization/Fruiting Temperatures: 84-86F/74-78F

The Ecuadorian strain of P. cubensis is perhaps one of the most popular strains for study due to very classic P. cubensis features. Long, thick, and meaty stalks with large dark caramel colored caps are characteristic of this strain.

P. Cubensis var. Ecuadorian produces large to medium sized fruits often reaching an average height of five inches. The developing primordia heads appear very dark red in color, lightening to a dark caramel color upon full maturity. Stalks are robust in nature, although not quite as thick as the Golden Teacher. Caps average 2-3 inches in diameter. Second and third flush fruits often reach heights of eight inches or more when cased and grown on grain based substrates

Average colonization times for this strain is 10-13 days for 1/2-pint cake style jars. Mycelium growth is usually noticeable around the sixth to seventh day after syringe inoculation. According to our European research associated, this strain traditionally takes longer than most to show the first signs of spore germination, however, once growth appears it will quickly colonize the remaining substrate. This strain performs very well both cased and uncased and is highly recommended for study by the novice mycologist due to very classic P. cubensis features.

This particular strain comes from the beautiful Andes of Peru and is a very robust strain that produces high yields of crops, which are known to be one of the more potent strains of P.cubensis available.

It performs very well indoors proving a high tolerance for less than ideal grow space conditions, therefore making it an ideal choice for beginners.

Psilocybe cubensis PES Hawaiian

Cultivation Difficulty: Easy
Potency: Moderate to Potent
Substrate: Dung, Straw, Various grains
Colonization/Fruiting Temperatures: 84-86F/74-78F

A strain originally from the Hawaii based spore retailer, Pacifica Exotic Spora. Psilocybe cubensis itself is not native to the Hawaiian Islands.

Average fruit body heights reached five to seven inches. The stalk is fleshy although not very robust. The cap color is caramel to dark red to the center. Average cap diameters are between one and three inches. The caps also display some rather pronounced spotting, which often remains present until full maturity. Colonization times are fast, often consuming 1/2 pints of spawn in just less than 13 days.

This strain is highly recommended for first time microscopy as this P. cubensis exhibits many of the "classic" characteristics commonly associated with the species.

It usually produces larger sized fruit bodies that somewhat resemble the Golden Teacher. It's not certain if there is a relation between the GTs and this one, but they do share some similar characteristics.