If you could build a lab...........

[Agent-Smith]

If you had the money to build a personal lab setup for studying cannabis, what types of equipment would you buy, and why.

[Joe King Park]

a cargo freighter and www.grow-go.nl
and the crew would be selected from C-W
and we would live in International Waters until we could afford to buy a Country

Now , how many containers could we run, roflmao
Joe

[joe6pack]

a gas spectrometer to study THC content and other terpines

dna sequencing equipment to study and catalogue the genome of each drug cannabis cultivar

cell culture equipment (agar making - sterlization - incubation) to make cell cultures instead of clones (think real tiny clones)

ultra centrifuge (with bubble bag attachment.... LOL)

Solar panels and maybe a small nuclear reactor to power everything on the cargo freighter that JKP aquired for the lab

oh and a bunch of really really nice glass bongs for lab staff

[LateShaftNBaggins]

a gas spectrometer to study THC content and other terpines

dna sequencing equipment to study and catalogue the genome of each drug cannabis cultivar

cell culture equipment (agar making - sterlization - incubation) to make cell cultures instead of clones (think real tiny clones)

ultra centrifuge (with bubble bag attachment.... LOL)

Solar panels and maybe a small nuclear reactor to power everything on the cargo freighter that JKP aquired for the lab

oh and a bunch of really really nice glass bongs for lab staff
All that stuff... And I'll pretend like I knew like it all existed before I read this..
LSnB

[coffinman]

tissue culture doesn't need expensive equipment. You just need the right hormones and you need to figure out the right ratio of auxin to cytokinin for cannabis plants. Other than that you are good to go.

[suzy cremecheese]

tissue culture doesn't need expensive equipment. You just need the right hormones and you need to figure out the right ratio of auxin to cytokinin for cannabis plants. Other than that you are good to go.

I remember reading an article about a guy that worked for a university that got busted with way too many plants and claimed he was using them for research and not selling them. He had, supposedly, developed a technique to grow plants from individual cells. I don't know of anyone that has perfected this with cannabis yet. Maybe someone should track this guy down. He might have the hormone recipe.

Needless to say he lost his job but the judge bought his story about research and no sales. He did time but alot less than normal.

If anyone can find a link based on that info I will be impressed.

[coffinman]

Suzy if you don't have auxin or cytokinin here is an easy home made remedy to try out:

Plant Tissue Culture for Home Gardeners
Micropropagtion is an important alternative to more conventional methods of plant propagation. It involves production of plants from very small plant parts (e.g. buds, nodes, leaf segments, root segments etc.), grown aseptically (free from any microorganism) in a container where the environment and nutrition can be controlled. The resultant plants are genetically identical to parent plants.
Whilst in a research laboratory such as that in the Department of Agronomy and Soil Science at UNE we use many high tech equipment to achieve plant production through tissue culture, it is important to note that many home gardeners and hobbyists could substitute the high tech equipment and instruments with ordinary house hold items.


Items Needed for Home Tissue Culture:
1 - A sterile still air cabinet used to transfer plants. A fish tank on its side makes an ideal transfer cabinet. Any perspex or glass chamber with dimensions of 50 cm (length), 40 cm (height) and 40 cm (depth) could easily be made into a transfer cabinet.
2 - A pressure cooker for sterilisation of media, instruments, water, paper towelling etc.
3 - Glass jars (baby food jars are excellent) and take away food containers with lids which can withstand the heat inside a pressure cooker are ideal vessels to use.
4 - Scalpel and forceps
5 - Paper towelling or even A4 white copy paper, cut to size, can be sterilised and used for a sterile cutting surface.
6 - A spirit lamp containing ethanol for flaming the instruments (avoid using Methanol as it is toxic!).
7 - Hand held spray bottle containing 70% alcohol solution to spray the transfer chamber and other surfaces.
8 - Dilute chlorine solution e.g. 1/4 dilution of the household bleach (e.g. White King) for use in surface sterilisation of plant material.
9 - Any skin disinfectant e.g. Hibitane (obtainable from any chemist shop).
10 - Media (see below)

Media Preparation
All the ingredients indicated below can be purchased using the super market, chemist and a health food shop.
1- Two cups of rain water
2- A quarter cup of sugar
3- Fertiliser stock: 1/2 tablespoon all purpose 10:10:10 (N.P.K.) water soluble fertiliser in 1L of water: use one cup of stock for this recipe.
4- Inositol tablets (500 mg): 1/2 tablet
5- Vitamin tablet with thiamine: 1/2 tablet- Any multivitamin tablet may be used.
6- Agar flakes: 4 tablespoons

This is the basic media. For preparation of multiplication and rooting media add 1/2 cup of coconut milk and 1/2 teaspoon of malt. Replacing the coconut milk with 1/2 cup of green tomato puree or 1/2 cup of freshly squeezed orange juice may produce different responses. Ensure that the pH of medium is always between 5 and 6 using narrow range pH indicator tape. Adjust pH if necessary, with acid e.g. Citric acid or base e.g bicarb soda.
Mix the ingredients in a saucepan and gently boil until the agar has dissolved, stirring continuously to stop the agar sticking and subsequently burning at the bottom of the pan. Dispense into empty glass jars, using a ladle, so that the medium is about 2 cm deep. Cover and process in a pressure cooker. Cook for 15 minutes after the pressure is reached (this will be achieved when the pressure valve starts letting steam out).


Sterilising Instruments and Other Items
Forceps and scalpels can be sterilised by being wrapped in Alfoil and cooked in the pressure cooker for 15 minutes. These items can also be sterilised by being washed in chlorine solution or being dipped in alcohol and flamed.
Sterile water is needed for rinsing plant material and sterile paper towelling to be used as a clean surface to work on. The manipulation can be accomplished on the paper. When the operation is completed, the towel can be discarded and a new sterile surface selected from the sterile supply. The water can be sterilised in glass jars. Place paper inside a paper bag and cook them in the pressure cooker above the water level. The bag will be wet on completion of sterilisation. Transfer the bag to an oven set at 80

[Agent-Smith]

nice CM! How about a gene splicer! Now that could be a really fun machine!!

[coffinman]

Now your talkin AS. We would also need a lab with the prerequisite gear, where we can isolate the gene of interest which we want to splice into our plant. Damn I can't wait for grad school.

[Green Supreme]

B-Rye says, A Roor

[suzy cremecheese]

CM,

Has that technique worked for you with cannabis?

I would want a flow cytometer and the equipment and expertise required to use it.

[Agent-Smith]

I would want a flow cytometer and the equipment and expertise required to use it.

What is it and what does it do?

[suzy cremecheese]

Its one of the more accurate instruments for determining the number of chromosomes in a cell.

[Organic Alchemist]

Ok give me....
supercritical carbon dioxide extractor

Gas chromatograph/ mass spectrometer for analysis of cannabinoids and volatile fragrance/flavor components

large greenhouse(s) for production

All you guys can handle the genetic work and I will take care of analysis and extraction protocol!
PEACE

[capt carnuba]

I just need some Rizla's and about 50 legal acres.

[Razz]

i'd buy some weed.

[vapor]

ahahaa definately a Roor!!!

[coffinman]

CM,

Has that technique worked for you with cannabis?

I would want a flow cytometer and the equipment and expertise required to use it.
I haven't tried it yet suzy but am planning on doing it this fall. I figure I can work on my girls and a legal plant at the same time so I can write up a paper for my plant bio class which I will be taking in the fall. It would be nice if it works out, if so I will start a thread on it so everyone can know how to do it.

[suzy cremecheese]

From what I understand the recipe may have to be tweaked from species to species and even variety to variety. Sounds like a great paper. Please keep us posted and let us know what recipe you find works.

[outdoordreams]

Exciting times we do live in..............I will definetly be watching this thread........

[Springs]

http://www.biobubble.com/biobubble_ppt/img123.html

something like that with separation between the soil and plant with poly and elastic stalk collar.

Beats irradiation!

[DOZEE]

bio-dome, several terarium's .. with different animals and plants..

[Brick Top]

Gas chromatograph/ mass spectrometer, dna sequencing equipment, cell culture equipment, ultra centrifuge, supercritical carbon dioxide extractor.


Located here.